Abstract
Key message
Improved compact shoot architecture of Osteospermum fruticosum Ri lines obtained through Rhizobium rhizogenes transformation reduces the need for chemical growth retardants.
Abstract
Compactness is for many ornamental crops an important commercial trait that is usually obtained through the application of growth retardants. Here, we have adopted a genetic strategy to introduce compactness in the perennial shrub Cape daisy (Osteospermum fruticosum Norl.). To this end, O. fruticosum was transformed using six different wild type Rhizobium rhizogenes strains. The most effective R. rhizogenes strains Arqua1 and ATCC15834 were used to create hairy root cultures from six Cape daisy genotypes. These root cultures were regenerated to produce transgenic Ri lines, which were analyzed for compactness. Ri lines displayed the characteristic Ri phenotype, i.e., reduced plant height, increased branching, shortened internodes, shortened peduncles, and smaller flowers. Evaluation of the Ri lines under commercial production conditions showed that similar compactness was obtained as the original Cape daisy genotypes treated with growth retardant. The results suggest that the use of chemical growth retardants may be omitted or reduced in commercial production systems of Cape daisy through implementation of Ri lines in future breeding programs.
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Acknowledgements
The authors would like to sincerely thank Ludo Decock, Marijn Parmentier and Gaël Decock for supplying the Cape daisy genotypes and offering the opportunity to conduct greenhouse experiments. We would also like to thank Kristien Janssens, Laurence Desmet, Magali Losschaert, and Roger Dobbelaere for the technical assistance. Special thanks to Mia Biebrouck and Nic Claerhout for the excellent management of greenhouse plants.
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This study was funded by the Flanders Agency for Innovation & Entrepreneurship (grant number 150889).
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SD, ED, EDK, JVH, and DG conceived and designed the experiments. SD performed the experiments. SD, ED, and EDK analyzed the data. SD wrote the manuscript; other authors provided editorial advice.
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Desmet, S., Dhooghe, E., De Keyser, E. et al. Compact shoot architecture of Osteospermum fruticosum transformed with Rhizobium rhizogenes. Plant Cell Rep 40, 1665–1678 (2021). https://doi.org/10.1007/s00299-021-02719-z
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DOI: https://doi.org/10.1007/s00299-021-02719-z