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Hypoxia enhances lignification and affects the anatomical structure in hydroponic cultivation of carrot taproot

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Hypoxia enhances lignification of carrot root.

Abstract

Hypoxia stress was thought to be one of the major abiotic stresses that inhibiting the growth and development of higher plants. The genes encoding the plant alcohol dehydrogenase (ADH-P) were induced when suffering hypoxia. To investigate the impact of hypoxia on the carrot root growth, carrot plants were cultivated in the hydroponics with or without aeration. Morphological characteristics, anatomical structure, lignin content, and the expression profiles of DcADH-P genes and lignin biosynthesis-related genes were measured. Six DcADH-P genes were identified from the carrot genome. The expression profiles of only three (DcADH-P1, DcADH-P2, and DcADH-P3) genes could be detected and the other three (DcADH-P4, DcADH-P5, and DcADH-P6) could not be detected when carrot cultivated in the solution without aeration. In addition, carrot roots had more lignin content, aerenchyma and less fresh weight when cultivated in the solution without aeration. These results suggested that hypoxia could enhance the lignification and affect anatomical structure of the carrot root. However, the expression levels of the genes related to lignin biosynthesis were down-regulated under the hypoxia. The enhancement of lignification may be the consequence of the structure changes in the carrot root. Our work was potentially helpful for studying the effect of hypoxia on carrot growth and may provide useful information for carrot hydroponics.

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Acknowledgements

The research was supported by the New Century Excellent Talents in University (NCET-11-0670); Jiangsu Natural Science Foundation (BK20130027); Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Feng Que, Guang-Long Wang, Kai Feng, Zhi-Sheng Xu, Feng Wang & Ai-Sheng Xiong

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  1. Feng Que
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  2. Guang-Long Wang
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  3. Kai Feng
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Correspondence to Ai-Sheng Xiong.

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Communicated by Maike Petersen.

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Que, F., Wang, GL., Feng, K. et al. Hypoxia enhances lignification and affects the anatomical structure in hydroponic cultivation of carrot taproot. Plant Cell Rep 37, 1021–1032 (2018). https://doi.org/10.1007/s00299-018-2288-3

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