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GID1 expression is associated with ovule development of sexual and apomictic plants

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Abstract

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BbrizGID1 is expressed in the nucellus of apomictic Brachiaria brizantha, previous to aposporous initial differentiation. AtGID1a overexpression triggers differentiation of Arabidopsis thaliana MMC-like cells, suggesting its involvement in ovule development.

Abstract

GIBBERELLIN-INSENSITIVE DWARF1 (GID1) is a gibberellin receptor previously identified in plants and associated with reproductive development, including ovule formation. In this work, we characterized the Brachiaria brizantha GID1 gene (BbrizGID1). BbrizGID1 showed up to 92% similarity to GID1-like gibberellin receptors of other plants of the Poaceae family and around 58% to GID1-like gibberellin receptors of Arabidopsis thaliana. BbrizGID1 was more expressed in ovaries at megasporogenesis than in ovaries at megagametogenesis of both sexual and apomictic plants. In ovules, BbrizGID1 transcripts were detected in the megaspore mother cell (MMC) of sexual and apomictic B. brizantha. Only in the apomictic plants, expression was also observed in the surrounding nucellar cells, a region in which aposporous initial cells differentiate to form the aposporic embryo sac. AtGID1a ectopic expression in Arabidopsis determines the formation of MMC-like cells in the nucellus, close to the MMC, that did not own MMC identity. Our results suggest that GID1 might be involved in the proper differentiation of a single MMC during ovule development and provide valuable information on the role of GID1 in sexual and apomictic reproduction.

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Acknowledgements

The authors acknowledge Dr. Perez-Amador who provided seeds of pAtGID1a:AtGID1a-GUS, pAtGID1b: AtGID1b-GUS and pAtGID1c:AtGID1c-GUS transgenic lines and Dr. Tucker who supplied seeds of pKNU:nlsYFP line. We also thank the assistance of Simona Masiero, Lilian Florentino and Júlio Rodrigues in the laboratory; Roberto Togawa and Priscila Grynberg in the bioinformatics analysis; and Joseane Padilha in the statistical analyses. This work was supported by the National Council for Scientific and Technological Development-CNPq (449636/2014-3-VTCC) and the Brazilian Agricultural Research Corporation-Embrapa (02140101400.00-VTCC). This work is part of Luciana Gomes Ferreira’s PhD thesis from Pós-Graduação em Biologia Molecular, University of Brasilia-UnB, Brazil, with fellowship from Coordination for the Improvement of Higher Level Personnel—University of Brasília, CAPES-UnB, which also provided a 1-year fellowship at Università degli Studi di Milano, Italy. Foundation for Research Support of the Federal District-FAPDF provided financial assistance for LGF’s participation in congresses.

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Authors and Affiliations

  1. Department of Biology, University of Brasília-UnB, Campus Darcy Ribeiro S/N–Asa Norte, Brasília, DF, 70910-900, Brazil

    Luciana Gomes Ferreira & Vera Tavares de Campos Carneiro

  2. Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB Av. W5 Norte, Caixa Postal 02372, Brasília, DF, 70770-917, Brazil

    Luciana Gomes Ferreira, Diva Maria de Alencar Dusi, André Southernman Teixeira Irsigler, Ana Cristina Meneses Mendes Gomes & Vera Tavares de Campos Carneiro

  3. Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milan, Italy

    Marta Adelina Mendes & Lucia Colombo

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  1. Luciana Gomes Ferreira
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  2. Diva Maria de Alencar Dusi
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  3. André Southernman Teixeira Irsigler
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  7. Vera Tavares de Campos Carneiro
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Correspondence to Vera Tavares de Campos Carneiro.

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Communicated by Xian Sheng Zhang.

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Ferreira, L.G., de Alencar Dusi, D.M., Irsigler, A.S.T. et al. GID1 expression is associated with ovule development of sexual and apomictic plants. Plant Cell Rep 37, 293–306 (2018). https://doi.org/10.1007/s00299-017-2230-0

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