Abstract
Key message
EaCHS1 functions in the tolerance of plantlets to salinity stress by maintaining ROS homeostasis.
Abstract
Chalcone synthase (CHS) is an essential enzyme in the biosynthesis of flavonoids. Expression of CHS is governed by a wide range of environmental stimuli, including UV light, pathogen attack, and circadian clocks. However, little research exists on the relationship between CHS and salinity stress. In this work, we constructed separate overexpression and RNA interference vectors of EaCHS1, and transferred them into tobacco. Overexpression of EaCHS1 increased the production of downstream flavonoids and the expressions of related genes in the phenylpropanoid pathway. It also improved resistance to salinity stress during seed germination and root development. In contrast, heterologous silencing of endogenous CHS in tobacco by a conserved EaCHS1 fragment had opposite effect. Together, our results indicated that changing the expression level of EaCHS1 in plants alters the accumulation of flavonoids and regulates plantlet tolerance to salinity stress by maintaining ROS homeostasis.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31201531) and (31372004). We would like to thank Dr. Zhang Zhijin, Dr. Ma Wei and Dr. Latifur Rehman for reading the manuscript and for their helpful suggestions.
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The authors declare that they have no conflict of interest.
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Communicated by C. H. Dong.
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299_2015_1751_MOESM1_ESM.tif
Figure S1. Quantitative real-time RT-PCR analysis of EaCHS1 expression in wild-type tobacco (WT), overexpression transgenic tobacco (OETT) lines, and RNAi transgenic tobacco (RTT) lines (TIFF 1061 kb)
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Figure S3. Quantitative real-time RT-PCR analysis of different core genes’ expressions in WT, OETT lines, and RTT lines.(TIFF 1406 kb)
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Figure S7. Measurements of chlorophyll content, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, catalase (CAT) activity, superoxide anion (O2 −) content and hydrogen peroxide (H2O2) levels in WT, OETT lines, and RTT lines treated with 0, 100, or 300 mM NaCl (TIFF 2945 kb)
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Lijuan, C., Huiming, G., Yi, L. et al. Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco. Plant Cell Rep 34, 885–894 (2015). https://doi.org/10.1007/s00299-015-1751-7
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DOI: https://doi.org/10.1007/s00299-015-1751-7