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OsMPK3 positively regulates the JA signaling pathway and plant resistance to a chewing herbivore in rice

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Abstract

Key message

Silencing OsMPK3 decreased elicited JA levels, which subsequently reduced levels of herbivore-induced trypsin protease inhibitors (TrypPIs) and improved the performance of SSB larvae, but did not influence BPH.

Abstract

Mitogen-activated protein kinases (MPKs) are known to play an important role in plant defense by transferring biotic and abiotic signals into programmed cellular responses. However, their functions in the herbivore-induced defense response in rice remain largely unknown. Here, we identified a MPK3 gene from rice, OsMPK3, and found that its expression levels were up-regulated in response to infestation by the larvae of the striped stem borer (SSB) (Chilo suppressalis), to mechanical wounding and to treatment with jasmonic acid (JA), but not to infestation by the brown planthopper (BPH) Nilaparvata lugens or to treatment with salicylic acid. Moreover, mechanical wounding and SSB infestation induced the expression of OsMPK3 strongly and quickly, whereas JA treatment induced the gene more weakly and slowly. Silencing OsMPK3 (ir-mpk3) reduced the expression of the gene by 50–70 %, decreased elicited levels of JA and diminished the expression of a lipoxygenase gene OsHI-LOX and an allene oxide synthase gene OsAOS1. The reduced JA signaling in ir-mpk3 plants decreased the levels of herbivore-induced trypsin protease inhibitors (TrypPIs) and improved the performance of SSB larvae, but did not influence BPH. Our findings suggest that the gene OsMPK3 responds early in herbivore-induced defense and can be regulated by rice plants to activate a specific and appropriate defense response to different herbivores.

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Abbreviations

JA:

Jasmonic acid

SA:

Salicylic acid

ET:

Ethylene

MPK:

Mitogen-activated protein kinase

MEKK:

MPK kinase kinase

MEK:

MPK kinase

WIPK:

Wound-induced protein kinase

SIPK:

Salicylic acid-induced protein kinase

TrypPIs:

Trypsin protease inhibitors

SSB:

Striped stem borer

BPH:

Brown planthopper

WT:

Wild type

qPCR:

Quantitative real-time PCR

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Acknowledgments

We thank Guoxin Zhou for his invaluable assistance with the experiments, and Emily Wheeler for editorial assistance. This study was jointly sponsored by the National Basic Research Program of China (2010CB126200), the National Natural Science Foundation of China (30871644), the National Program of Transgenic Variety Development of China (2011ZX08001-001), and the China Agriculture Research System (CARS-01-21).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Institute of Entomology, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China

    Qi Wang & Guren Zhang

  2. State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou, 310029, China

    Jiancai Li, Lingfei Hu, Tongfang Zhang & Yonggen Lou

Authors
  1. Qi Wang
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  2. Jiancai Li
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  3. Lingfei Hu
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  4. Tongfang Zhang
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  5. Guren Zhang
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Corresponding author

Correspondence to Yonggen Lou.

Additional information

Communicated by M. Petersen.

A contribution to the Special Issue: Plant Hormone Signaling.

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Wang, Q., Li, J., Hu, L. et al. OsMPK3 positively regulates the JA signaling pathway and plant resistance to a chewing herbivore in rice. Plant Cell Rep 32, 1075–1084 (2013). https://doi.org/10.1007/s00299-013-1389-2

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  • DOI: https://doi.org/10.1007/s00299-013-1389-2

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