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Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin

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Abstract

A single-chain variable fragment antibody (scFv) against plumbagin (PL) accumulated the PL production in the hairy roots of Plumbago zeylanica. Recombinant Agrobacterium rhizogenes (ATCC 15834) containing an scFv gene against PL (PL-scFv) were obtained through triparental mating and transformed into P. zeylanica to induce PL-scFv protein in the hairy roots. Up to 40 μg recombinant PL-scFv were expressed per milligram of soluble protein in transgenic P. zeylanica hairy root cultures. The mean PL content obtained from transgenic hairy roots (12.24 μg/100 mg dry weight) exhibited 2.2 times higher than those obtained from wild-type (5.48 μg/100 mg dry weight). The high correlation between the PL-scFv expression level and PL content of the recombinant plants suggested that the PL biosynthesis pathway had been modulated by the expression of PL-scFv protein in the hairy roots of P. zeylanica.

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Acknowledgments

We appreciate for the technical supports from the Research Support Center, Graduate School of Medical Sciences, Kyushu University. This work was funded by the Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists. The research in this paper was also supported, in part, by a Grant in Aid from the Japan Society for the Promotion of Science Asian CORE Program; the Ministry of Education, Culture, Sports, Science, and Technology of Japan; and the National Center for Genetic and Biotechnology (BIOTEC), Thailand.

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Authors and Affiliations

  1. Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Seiichi Sakamoto, Benyakan Pongkitwitoon, Hiroyuki Tanaka & Satoshi Morimoto

  2. Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Waraporn Putalun

  3. Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, 85 Warinchamrap, Ubon Ratchathani, 34190, Thailand

    Thaweesak Juengwatanatrakul

  4. Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan

    Yukihiro Shoyama

Authors
  1. Seiichi Sakamoto
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  2. Waraporn Putalun
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  3. Benyakan Pongkitwitoon
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  4. Thaweesak Juengwatanatrakul
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  5. Yukihiro Shoyama
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  6. Hiroyuki Tanaka
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  7. Satoshi Morimoto
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Corresponding author

Correspondence to Hiroyuki Tanaka.

Additional information

Communicated by Q. Zhao.

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Sakamoto, S., Putalun, W., Pongkitwitoon, B. et al. Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin. Plant Cell Rep 31, 103–110 (2012). https://doi.org/10.1007/s00299-011-1143-6

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  • DOI: https://doi.org/10.1007/s00299-011-1143-6

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