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Proteomic analysis of somatic embryogenesis in Valencia sweet orange (Citrus sinensis Osbeck)

  • Physiology and Biochemistry
  • Published:
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Abstract

Two dimensional gel electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was employed to study the somatic embryogenesis (SE) in Valencia sweet orange (Citrus sinensis Osbeck). Twenty-four differentially expressed proteins were identified at five time points of citrus SE (0, 1, 2, 3, 4 weeks after embryo initiation) covering globular, heart/torpedo and cotyledon-shaped embryo stages. The general expression patterns for these proteins were consistent with those appeared at 4 weeks of citrus SE. The most striking feature of our study was that five proteins were predicted to be involved in glutathione (GSH) metabolism and anti-oxidative stress, and they exhibited different expression patterns during SE. Based on that oxidative stress has been validated to enhance SE, the preferential representation for anti-oxidative proteins suggests that they could have a developmental role in citrus SE. Some proteins involved in cell division, photosynthesis and detoxification were also identified, and their possible roles in citrus SE were discussed.

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Abbreviations

2-DE:

Two-dimensional gel electrophoresis

ACN:

Acetonitril

CBB:

Coomassie brilliant blue

CHAPS:

3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonic acid

DTT:

dl-Dithiothreitol

EST:

Expressed sequence tag

GSH:

Glutathione

GST:

Glutathione-S-transferase

MALDI-TOF:

Matrix-assisted laser desorption ionization time-of-flight

MS:

Mass spectrum

PEBP:

Phosphatidylethanolamine-binding family protein

PHGPx:

Phospholipid hydroperoxide glutathione peroxidase

ROS:

Reactive oxygen species

SE:

Somatic embryogenesis

TFA:

Trifluoroacetic acid

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Acknowledgments

We thank Prof. S. Xiao and Dr. Q. Xu for their critical review of the manuscript. We also thank Shanghai Applied Protein Technology Co. Ltd for the technology support. The research was financially supported by the National Natural Science Foundation of China (No. 30570973, 30830078) and the Ministry of Education of China (IRT0548).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China

    Zhiyong Pan, Rui Guan, Shiping Zhu & Xiuxin Deng

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  1. Zhiyong Pan
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Correspondence to Xiuxin Deng.

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Communicated by P. Lakshmanan.

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Pan, Z., Guan, R., Zhu, S. et al. Proteomic analysis of somatic embryogenesis in Valencia sweet orange (Citrus sinensis Osbeck). Plant Cell Rep 28, 281–289 (2009). https://doi.org/10.1007/s00299-008-0633-7

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