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Regeneration of roots from callus reveals stability of the developmental program for determinate root growth in Sonoran Desert Cactaceae

  • Cell Biology and Morphogenesis
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Abstract

In some Sonoran Desert Cactaceae the primary root has a determinate root growth: the cells of the root apical meristem undergo only a few cell division cycles and then differentiate. The determinate growth of primary roots in Cactaceae was found in plants cultivated under various growth conditions, and could not be reverted by any treatment tested. The mechanisms involved in root meristem maintenance and determinate root growth in plants remain poorly understood. In this study, we have shown that roots regenerated from the callus of two Cactaceae species, Stenocereus gummosus and Ferocactus peninsulae, have a determinate growth pattern, similar to that of the primary root. To demonstrate this, a protocol for root regeneration from callus was established. The determinate growth pattern of roots regenerated from callus suggests that the program of root development is very stable in these species. These findings will permit future analysis of the role of certain Cactaceae genes in the determinate pattern of root growth via the regeneration of transgenic roots from transformed calli.

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Abbreviations

BA:

6-Benzylaminopurine

CIM:

Callus induction medium

CIMs:

Callus induction media

MS:

Murashige and Skoog medium

NAA:

1-Naphthaleneacetic acid

Nx :

NAA x mg/l

NxBy :

NAA x mg/l, BA y mg/l

RAM:

Root apical meristem

RRM:

Root regeneration medium

RRMs:

Root regeneration media

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Acknowledgments

The authors thank S. Napsucialy-Mendivil for excellent technical assistance, A. Martínez for computer assistance, N. Doktor for help with figures, M. Potapova and M. Matvienko for critical reading of the manuscript. This work was partially supported by DGAPA-PAPIIT (Universidad Nacional Autónoma de México) project IN227206.

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Authors and Affiliations

  1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A. P. 510-3, C. P. 62250, Cuernavaca, Morelos, Mexico

    Svetlana Shishkova, Edith García-Mendoza, Vicente Castillo-Díaz, Norma E. Moreno & Joseph G. Dubrovsky

  2. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, A. P. 565-A, C. P. 62250,, Cuernavaca, Morelos, Mexico

    Jesús Arellano

Authors
  1. Svetlana Shishkova
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  2. Edith García-Mendoza
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  3. Vicente Castillo-Díaz
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  4. Norma E. Moreno
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  5. Jesús Arellano
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  6. Joseph G. Dubrovsky
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Corresponding author

Correspondence to Svetlana Shishkova.

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Communicated by W. Harwood

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Shishkova, S., García-Mendoza, E., Castillo-Díaz, V. et al. Regeneration of roots from callus reveals stability of the developmental program for determinate root growth in Sonoran Desert Cactaceae. Plant Cell Rep 26, 547–557 (2007). https://doi.org/10.1007/s00299-006-0269-4

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  • DOI: https://doi.org/10.1007/s00299-006-0269-4

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