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Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines

  • Genetic Transformation and Hybridization
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Abstract

Somatic embryos of soybean [Glycine max (L.) Merrill] have been used to generate transgenic plants by particle bombardment. The induction and proliferation of somatic embryos from immature cotyledons are dependent on the genotype of the cultivar. Whereas somatic embryogenesis and plant regeneration are inefficient in most cultivars, they are efficient in the cultivar Jack. We previously established a breeding line, QF2, by the integration of null mutations of each subunit of the major seed storage proteins glycinin and β-conglycinin, but the embryogenic response of this line is insufficient to allow efficient transformation. We have now backcrossed QF2 to cultivar Jack in order to combine the null traits with competence for somatic embryogenesis. The backcrossed breeding lines selected on the basis of the absence of the major storage proteins exhibited an improved capacity for the induction and proliferation of somatic embryos compared with that of QF2. The induced somatic embryogenic tissue of these breeding lines was successfully used for the production of transgenic plants by particle bombardment. These results also indicate that somatic embryogenesis in soybean is genetically controlled and inherited in a manner independent of the null traits of the major seed storage proteins.

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Acknowledgments

This work was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN). We thank Y. Niwa for providing the sGFP(S65T) reporter gene.

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Authors and Affiliations

  1. Ehime Women’s College, 421 Ibuki, Uwajima, Ehime, 798-0025, Japan

    Yoichi Kita & Masahiko Kitayama

  2. National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-8555, Japan

    Keito Nishizawa & Masao Ishimoto

  3. National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi, Kumamoto, 861-1192, Japan

    Masakazu Takahashi

Authors
  1. Yoichi Kita
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  2. Keito Nishizawa
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  3. Masakazu Takahashi
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  4. Masahiko Kitayama
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  5. Masao Ishimoto
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Corresponding author

Correspondence to Masao Ishimoto.

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Communicated by F. Sato

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Kita, Y., Nishizawa, K., Takahashi, M. et al. Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines. Plant Cell Rep 26, 439–447 (2007). https://doi.org/10.1007/s00299-006-0245-z

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  • DOI: https://doi.org/10.1007/s00299-006-0245-z

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