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An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Ham.

  • Cell Biology and Morphogenesis
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Abstract

An efficient, cyclic, two-step protocol for micropropagation of medicinal tree, Crataeva nurvala has been successfully developed, which can be employed at a commercial scale. Nodal explants from 30-year-old tree when cultured on MS medium supplemented with 2.22 μM BAP produced multiple shoots, which elongated satisfactorily on the same medium. Nodal and leaf explants from in vitro regenerated microshoots too developed shoots, thus making the process recurrent. In 6-month duration, owing to the recurring nature of the protocol, over 5400 shoots could be produced from a single nodal explant from the adult tree. Addition of casein hydrolysate significantly increased the average number of shoots per explant. Maximum number of shoots regenerated on medium supplemented with 100 mg l−1 casein hydrolysate. Shoots could be rooted on 1/2 MS supplemented with 0.11 and 0.54 μM NAA. Regenerated plantlets were acclimatized and successfully transplanted to soil.

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Abbreviations

BAP:

6-Benzyl aminopurine

CH:

Casein hydrolysate

GA3 :

Gibberellic acid

MS:

Murashige and Skoog (1962)

NAA:

α-Naphthalene acetic acid

WPM:

Woody plant medium

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Author notes

  1. Neetika Walia

    Present address: The Energy and Resources Institute (TERI), Darbari Seth Block, India Habitat Place, Lodhi Road, Delhi, 110003, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Neetika Walia, Amandeep Kaur & Shashi B. Babbar

Authors
  1. Neetika Walia
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  2. Amandeep Kaur
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  3. Shashi B. Babbar
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Corresponding author

Correspondence to Neetika Walia.

Additional information

Communicated by S. A. Merkle

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Walia, N., Kaur, A. & Babbar, S.B. An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Ham.. Plant Cell Rep 26, 277–284 (2007). https://doi.org/10.1007/s00299-006-0239-x

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  • DOI: https://doi.org/10.1007/s00299-006-0239-x

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