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A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response

  • Genetics and Genomics
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Abstract

To understand the transcriptional regulatory mechanism of host genes during the activation of defense responses in rice, we isolated WRKY transcription factors whose expressions were altered upon attack of the fungal pathogen Magnaporthe grisea, the causal agent of the devastating rice blast disease. A systematic expression analysis of OsWRKYs (Oryza sativa L. WRKYs) revealed that among 45 tested genes the expression of 15 genes was increased remarkably in an incompatible interaction between rice and M. grisea. Twelve of the M. grisea-inducible OsWRKY genes were also differentially regulated in rice plants infected with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). In experiments with defense signaling molecules, the expression of two genes, OsWRKY45 and OsWRKY62, was increased in salicylic acid (SA)-treated leaves and the expression of three genes, OsWRKY10, OsWRKY82, and OsWRKY85 was increased by jasmonic acid (JA) treatment. OsWRKY30 and OsWRKY83 responded to both SA- and JA treatments. The expression profiles suggest that a large number of WRKY DNA-binding proteins are involved in the transcriptional activation of defense-related genes in response to rice pathogens.

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Acknowledgements

We thank Dr. Jong-Min Nam (California Institute of Technology, USA) for advice concerning phylogenetic analysis. This work was supported, in part, by grants from the Biogreen 21 program, Rural Development Administration; from the SRC program of MOST/KOSEF (R11-2000-081) through the Plant Metabolism Research Center; from the Crop Functional Genomics Center (CFGC) of the 21st Century Frontier Research Program (CG1422); from the Agricultural R&D Promotion Center of the Ministry of Agriculture and Forestry; and from the BK21 program, Ministry of Education.

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  1. Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Yongin, 449-701, Korea

    Hak-Seung Ryu, Muho Han, Sang-Kyu Lee, Jung-Il Cho, Nayeon Ryoo, Youn-Hyung Lee, Seong Hee Bhoo, Tae-Ryong Hahn & Jong-Seong Jeon

  2. Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, 441-707, Korea

    Sunggi Heu

  3. Department of Plant Pathology, The Ohio State University, 201 Kottman Hall, 2021 Coffey Road, Columbus, OH, 43210, USA

    Guo-Liang Wang

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  1. Hak-Seung Ryu
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Ryu, HS., Han, M., Lee, SK. et al. A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response. Plant Cell Rep 25, 836–847 (2006). https://doi.org/10.1007/s00299-006-0138-1

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