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Complete sequence and organization of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome

  • Genetics and Genomics
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Abstract

The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed (DQ119058). The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,879 and 18,274 bp, respectively. The presence and relative positions of 113 genes (76 peptide-encoding genes, 30 tRNA genes, four rRNA genes, and three conserved open reading frames) were identified. The major portion (55.76%) of the C. sativus chloroplast genome consisted of gene-coding regions (49.13% protein coding and 6.63% RNA regions; 27.81% LSC, 9.46% SSC and 18.49% IR regions), while intergenic spacers (including 20 introns) made up 44.24%. The overall G-C content of C. sativus chloroplast genome was 36.95%. Sixteen genes contained one intron, while two genes had two introns. The expansion/contraction manner of IR at IRb/LSC and IR/SSC border in Cucumis was similar to that of Lotus and Arabidopsis, and the manner at IRa/LSC was similar to Lotus and Nicotiana. In total, 56 simple sequence repeats (more than 10 bases) were identified in the C. sativus chloroplast genome.

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Authors and Affiliations

  1. Biological Function Research Team, Korea Research Institute of Chemical Technology, P.O Box 107, Yuseong, Daejeon, 305-600, Korea

    Jin-Seog Kim, Jung-Ae Lee, Kwang-Hoon Oh & Kwang Yun Cho

  2. Eugentech Inc, BVC 112, KRIBB, 52 Eoun-dong, Yuseong, Daejon, 305-806, Korea

    Jong Duk Jung & Hyun-Woo Park

  3. Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, 52 Eoun-dong, Yuseong, Daejon, 305-806, Korea

    Won-Joong Jeong & Jang Ryol Liu

  4. Department of Biology Education, Chonnam National University, Kwangju, 500-757, Korea

    Dong-Woog Choi

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  1. Jin-Seog Kim
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  2. Jong Duk Jung
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  3. Jung-Ae Lee
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Correspondence to Jin-Seog Kim.

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Communicated by I. S. Chung

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Kim, JS., Jung, J.D., Lee, JA. et al. Complete sequence and organization of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome. Plant Cell Rep 25, 334–340 (2006). https://doi.org/10.1007/s00299-005-0097-y

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  • DOI: https://doi.org/10.1007/s00299-005-0097-y

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