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Transformation of Actinidia eriantha: A potential species for functional genomics studies in Actinidia

  • Genetic Transformation and Hybridization
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Abstract

Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (β-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.

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Acknowledgements

This research was funded by New Zealand Foundation for Research, Science, and Technology, contract number C06X0207. The authors thank Shavindra Bajaj and Bart Janssen for critical reading of the manuscript, Alan Seal for providing winter dormant canes of A. eriantha, Julie Nicholls and Wade Wadasinghe for their efforts in maintaining plants in containment greenhouse, and Martin Heffer and Tim Holmes for their kind help with photography.

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Author notes

  1. Yidong Ran

    Present address: PIR Victoria, La Trobe University, Melbourne, Australia

Authors and Affiliations

  1. The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand

    Tianchi Wang, Yidong Ran, Ross G. Atkinson, Andrew P. Gleave & Dan Cohen

Authors
  1. Tianchi Wang
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  2. Yidong Ran
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  3. Ross G. Atkinson
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  4. Andrew P. Gleave
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  5. Dan Cohen
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Correspondence to Tianchi Wang.

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Communicated by F. Sato

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Wang, T., Ran, Y., Atkinson, R.G. et al. Transformation of Actinidia eriantha: A potential species for functional genomics studies in Actinidia . Plant Cell Rep 25, 425–431 (2006). https://doi.org/10.1007/s00299-005-0080-7

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  • DOI: https://doi.org/10.1007/s00299-005-0080-7

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