Abstract
To develop an FLP-FRT recombination system- (derived from 2 μ plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the β-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.
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Abbreviations
- GUS:
-
β-Glucuronidase
- CaMV 35S:
-
Cauliflower Mosaic Virus 35S promoter
- hpt :
-
Hygromycin phosphotransferase gene
- nos3′:
-
Transcription termination sequence of nopaline synthase gene
- npt :
-
Neomycin phosphotransferase gene
- ubi-1 :
-
Maize ubiquitin-1 promoter
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Acknowledgements
This work was funded by the Arkansas Biosciences Institute and Arkansas Rice Research Board. We thank Drs. T. K. Hodges and David W. Ow for providing the plasmids.
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Communicated by G.C. Phillips
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Radhakrishnan, P., Srivastava, V. Utility of the FLP-FRT recombination system for genetic manipulation of rice. Plant Cell Rep 23, 721–726 (2005). https://doi.org/10.1007/s00299-004-0876-x
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DOI: https://doi.org/10.1007/s00299-004-0876-x