Skip to main content
SpringerLink
Log in

Methylmalonate-semialdehyde dehydrogenase is induced in auxin-stimulated and zinc-stimulated root formation in rice

  • Physiology and Biochemistry
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Proteins induced in rice by auxin and zinc were determined by proteome analysis. Cultured suspension cells of rice were treated with 2,4-dichlorophenoxyacetic acid and ZnSO4 and then proteins were separated by two-dimensional polyacrylamide gel electrophoresis; seven proteins were found to be induced by auxin and zinc. Of these seven, methylmalonate-semialdehyde dehydrogenase (MMSDH) was elevated by treatment with auxin alone. MMSDH was detected in cultured suspension cells, root and leaf sheath, but not in leaf blades. MMSDH responded to auxin and gibberellin, but did not respond to brassinolide and cytokinin. Furthermore, the amount of MMSDH in slr1, a constitutive gibberellin response mutant, was 2-fold that of wild type. MMSDH mRNA and protein were stimulated in root formation induced by auxin and/or zinc over a 4-week period. These results suggest that MMSDH may be necessary for root formation in rice induced by auxin and/or zinc.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1A,B
Fig. 2A,B
Fig. 3
Fig. 4
Fig. 5A,B
Fig. 6A–D
Fig. 7A,B
Fig. 8A–C

Similar content being viewed by others

Abbreviations

BA :

6-Benzylaminopurine

BL :

Brassinolide

CBB :

Coomassie Brilliant Blue

2,4-D :

2,4-Dichlorophenoxyacetic acid

GA :

Gibberellic acid

IAA :

Indole-3-acetic acid

MALDI-TOF MS :

Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry

MMSDH :

Methylmalonate-semialdehyde dehydrogenase

2D-PAGE :

Two-dimensional polyacrylamide gel electrophoresis

PVDF :

Polyvinylidene difluoride

References

  • Abel S, Theologis A (1996) Early genes and auxin action. Plant Physiol 111:9–17

    PubMed  Google Scholar 

  • Becker D, Hedrich R (2002) Channelling auxin action: modulation of ion transport by indole-3-acetic acid. Plant Mol Biol 49:349–356

    Article  CAS  PubMed  Google Scholar 

  • Cakmak I, Marschner H, Bangerth F (1989) Effect of zinc nutritional status on growth, protein metabolism and leaves of indole-3-acetic acid and other phytohormones in bean (Phaseolus vulgaris L.). J Exp Bot 40:405–412

    CAS  Google Scholar 

  • Casimiro I, Marchant A, Bhalerao RP, Beeckman T, Dhooge S, Swarup R, Graham N, Inzé D, Sandberg G, Casero PJ, Bennett M (2001) Auxin transport promotes Arabidopsis lateral root initiation. Plant Cell 13:843–852

    Google Scholar 

  • Chen JG (2001) Dual auxin signaling pathways control cell elongation and division. J Plant Growth Regul 20:255–264

    CAS  Google Scholar 

  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159

    CAS  PubMed  Google Scholar 

  • Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel. J Biol Chem 252:1102–1106

    CAS  PubMed  Google Scholar 

  • Curie C, Axelos M, Bardet C, Atanassova R, Chaubet N, Lescure B (1993) Modular organization and developmental activity of an Arabidopsis thaliana EF-1α gene promoter. Mol Gen Genet 238:428–436

    CAS  Google Scholar 

  • Fry SC (1998) Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals. Biochem J 332:507–515

    CAS  PubMed  Google Scholar 

  • Hagen G, Guilfoyle T (2002) Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol Biol 49:373–385

    Article  CAS  PubMed  Google Scholar 

  • Hlinkva E, Ruzickova C (2000) Gene expression of soybean calli culture affected by auxinoids with various chemical structure. Soybean Genet Newslett 27

  • Hossain B, Hirata N, Nagatomo Y, Akashi R, Takaki H (1997) Internal zinc accumulation is correlated with increased growth in rice suspension culture. J Plant Growth Regul 16:239–243

    CAS  Google Scholar 

  • Hossain B, Hirata N, Nagatomo Y, Suiko M, Takaki H (1998) Zinc nutrition and level of endogenous indole-3-acetic acid in radish shoots. J Plant Nutr 21:1113–1128

    CAS  Google Scholar 

  • Iino M, Long C, Wang X (2001) Auxin- and abscisic acid-dependent osmoregulation in protoplasts of Phaseolus vulgaris pulvini. Plant Cell Physiol 42:1219–1227

    Article  CAS  Google Scholar 

  • Ikeda A, Ueguchi-Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, Yamaguchi J (2001) Slender rice, a constitutive gibberellin response mutant, is caused by null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. Plant Cell 13:999–1010

    CAS  PubMed  Google Scholar 

  • Imai R, Chang L, Ohta A, Bray EA, Takagi M (1996) A lea-class gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae. Gene 170:243–248

    Article  CAS  PubMed  Google Scholar 

  • Kamo K, Blowers A (1999) Tissue specificity and expression levels of gusA under rolD, mannopine synthase and translation elongation factor 1 subunit β promoters in transgenic Gladiolus plants. Plant Cell Rep 18:809–815

    CAS  Google Scholar 

  • Kato N, Esaka M (1999) Changes in ascorbate oxidase gene expression and ascorbate levels in cell division and cell elongation in tobacco cells. Physiol Plant 105:321–329

    Article  CAS  Google Scholar 

  • Kedishvili NY, Popov KM, Rougraff PM, Zhao Y, Crabb DW, Harris RA (1992) CoA-dependent methylmalonate-semialdehyde dehydrogenase, a unique member of the aldehyde dehydrogenase superfamily. J Biol Chem 267:19724–19729

    CAS  PubMed  Google Scholar 

  • Kende H, Zeevaart JAD (1997) The five “classical” plant hormones. Plant Cell 9:1197–1210

    CAS  Google Scholar 

  • Kirsch C, Takamiya-Wik M, Reinold S, Hahlbrock K, Somssich IE (1997) Rapid, transient, and highly localized induction of plastidial omega-3-fatty acid desaturase mRNA at fungal infection sites in Petrosilenum crispum. Proc Natl Acad Sci USA 94:2079–2084

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    PubMed  Google Scholar 

  • Laskowski MJ, Williams ME, Nusbaum HC, Sussex IM (1995) Formation of lateral root meristems is a two-stage process. Development 121:3303–3310

    CAS  PubMed  Google Scholar 

  • Lee S, Woffenden BJ, Beers EP, Roberts AW (2000) Expansion of cultured Zinnia mesophyll cells in response to hormones and light. Physiol Plant 108:216–222

    Article  CAS  Google Scholar 

  • Marschner H (1986) Mineral nutrition of higher plants. Academic, New York

  • Matsubara S, Takizawa T, Takayama T, Iwasaki R, Minakami H, Sato I (2001) Cytochemically detectable glucose-6-phosphate dehydrogenase is present in human fetal membrane chorion leave trophoblasts. Placenta 22:613–616

    CAS  PubMed  Google Scholar 

  • Mehdy MC, Sharma YK, Sathasivan K, Bays NW (1996) The role of activated oxygen species in plant disease resistance. Physiol Plant 98:365–374

    Article  CAS  Google Scholar 

  • Morelli JK, Shewmaker CK, Vayda ME (1994) Biphasic stimulation of translational activity correlates with induction of translation elongation factor 1 subunit β upon wounding in potato tubers. Plant Physiol 106:897–903

    CAS  PubMed  Google Scholar 

  • Natera SH, Guerreiro N, Djordjevic MA (2000) Proteome analysis of differentially displayed proteins as a tool for the investigation of symbiosis. Mol Plant Microbe Interact 13:995–1009

    PubMed  Google Scholar 

  • O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    PubMed  Google Scholar 

  • Pandey A, Mann M (2000) Proteomics to study genes and genomes. Nature 405:837–846

    PubMed  Google Scholar 

  • Paul RU, Holk A, Scherer GFE (1998) Fatty acids and lysophospholipids as potential second messengers in auxin action. Rapid activation of phospholipase A2 activity by auxin in suspension-cultured parsley and soybean cells. Plant J 16:601–611

    Article  CAS  Google Scholar 

  • Pearson WR, Lipman DJ (1998) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448

    Google Scholar 

  • Reid JB, Murfet IC, Potts WC (1983) Internode length in Pisum. II. Additional information on the relationship and action of loci Le, La, Cry, Na and Lm. J Exp Bot 34:349–364

    Google Scholar 

  • Romo S, Labrador E, Dopico B (2001) Water stress-regulated gene expression in Cicer arietinum seedling and plants. Plant Physiol Biochem 39:1017–1026

    CAS  Google Scholar 

  • Rossignol M (2001) Analysis of the plant proteome. Curr Opin Biotechnol 12:131–134

    Article  CAS  PubMed  Google Scholar 

  • Saeki Y, Yasukouchi A, Nagatomo Y, Takaki H (2000) Distinctive expression of a zinc-binding protein in rice callus growth with high zinc concentration. Soil Sci Nutr 46:209–216

    CAS  Google Scholar 

  • Sasaki T, Matsumoto T, Yamamoto K, Sakata K, Baba T, Katayose Y, Wu J, Niimura Y, Cheng Z, Nagamura Y, Antonio BA, Kanamori H, Hosokawa S, Masukawa M, Arikawa K, Chiden Y, Hayashi M, Okamoto M, Ando K, Aoki H, Arita K, Hamada M, Harada C, Hijishita S, Honda M, Ichikawa Y, Idonuma A, Iijima M, Ikeda M, Ikeno M, Ito S, Ito T, Ito Y, Iwabuchi A, Kamiya K, Karasawa W, Katagiri S, Kikuta A, Kobayashi N, Kono I, Machita K, Maehara T, Mizuno H, Mizubayashi T, Mukai Y, Nagasaki H, Nakashima M, Nakama Y, Nakamichi Y, Nakamura M, Namiki N, Negishi M, Ohta I, Ono N, Saji S, Sakai K, Shibata M, Shimokawa T, Shomura A, Song J, Takazaki Y, Terasawa K, Tsuji K, Waki K, Yamagata H, Yamane H, Yoshiki S, Yoshihara R, Yukawa K, Zhong H, Iwama H, Endo T, Ito H, Hahn JH, Kim H, Eun M, Yano M, Jiang J, Gojobori T (2002) The genome sequence and structure of rice chromosome 1. Nature 420:312–316

    Article  CAS  PubMed  Google Scholar 

  • Schopfer P (2001) Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: implications for the control of elongation growth. Plant J 28:679–688

    Article  CAS  PubMed  Google Scholar 

  • Sekimoto H, Hoshi M, Nomura T, Yokota T (1997) Zinc deficiency affects the levels of endogenous gibberellins in Zea mays L. Plant Cell Physiol 38:1087–1090

    CAS  Google Scholar 

  • Skoog F (1940) Relationships between zinc and auxin in the growth of higher plant. Am J Bot 27:939–951

    CAS  Google Scholar 

  • Suge H, Takahashi S, Arita S, Takaki H (1986) Gibberellin relationships in zinc-deficient plants. Plant Cell Physiol 27:1005–1012

    CAS  Google Scholar 

  • Sweetlove LJ, Heazlewood JL, Herald V, Holtzapffel R, Day DA, Millar AH (2002) The impact of oxidative stress on Arabidopsis mitochondria. Plant J 32:891–904

    Article  CAS  PubMed  Google Scholar 

  • Swire-Clark GA, Marcotte WR Jr (1999) The wheat LEA protein functions as an osmoprotective molecule in Saccharomyces cerevisiae. Plant Mol Biol 39:117–128

    Article  CAS  PubMed  Google Scholar 

  • Tsui C (1948) The role of zinc in auxin synthesis in tomato plants. Am J Bot 35:172–179

    CAS  Google Scholar 

  • Van Gestelen P, Asard H, Caubergs RJ (1997) Solubilization and separation of a plant plasma membrane NADPH-O2 − synthase from other NAD(P)H oxidoreductases. Plant Physiol 115:543–550

    CAS  PubMed  Google Scholar 

  • Yoshida KT, Kuboyama T (1997) Isolation of sexual reproduction-related genes in rice by simplified differential display. Rice Genet Newslett 14:126–129

    Google Scholar 

Download references

Acknowledgements

We thank Dr. M. Matsuoka for the gift of rice plant materials. This work was supported by a grant from a MAFF Rice Genome Project PR-1215.

Author information

Authors and Affiliations

  1. Faculty of Agriculture, Miyazaki University, Miyazaki, 889-2192, Japan

    Keiko Oguchi & Shoichiro Akao

  2. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Naoki Tanaka & Setsuko Komatsu

Authors
  1. Keiko Oguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naoki Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Setsuko Komatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shoichiro Akao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Setsuko Komatsu.

Additional information

Communicated by H. Uchimiya

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oguchi, K., Tanaka, N., Komatsu, S. et al. Methylmalonate-semialdehyde dehydrogenase is induced in auxin-stimulated and zinc-stimulated root formation in rice. Plant Cell Rep 22, 848–858 (2004). https://doi.org/10.1007/s00299-004-0778-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-004-0778-y

Keywords

Search

Navigation