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Hypermethylation of retrotransposons in the liverwort Marchantia paleacea var. diptera

  • Genetics and Genomics
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Abstract

Three DNA fragments encoding part of a reverse transcriptase similar to that of Ty1-copia retrotransposons were isolated from suspension-cultured cells of the liverwort Marchantia paleacea var. diptera. Digestion of total DNA with methylation-sensitive and -insensitive isoschizomers revealed that, like those of the subtelomeric region, CpG sequences in retrotransposon genes were hypermethylated. The methylation status and expression of these retrotransposons was less affected by 5-azadeoxycytidine treatment compared to the subtelomeric region, which was considerably demethylated.

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Fig. 1A–C
Fig. 2A, B
Fig. 3A–D
Fig. 4A, B

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Abbreviations

5-azadC :

5-Azadeoxycytidine

MS :

Murashige and Skoog medium

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Acknowledgements

The authors would like to thank Dr. M. Yamaguchi at Hiroshima University, Japan, for providing thalli of M. paleacea var. diptera. We also thank to Dr. N. Saga at Hokkaido University, Japan, for providing thalli of P. yezoensis. This work was supported by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN; to H.T.).

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Authors and Affiliations

  1. Graduate School of Science and Technology, Kumamoto University, Kurokami, 860–8555, Kumamoto, Japan

    T. Fukuda & H. Takano

  2. Department of Biological Science, Faculty of Science, Kumamoto University, Kurokami, 860–8555, Kumamoto , Japan

    M. Sakai & K. Ono

  3. Center for Marine Environment Studies, Kumamoto University, Kumamoto, 860–8555, Japan

    S. Takio

Authors
  1. T. Fukuda
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  2. M. Sakai
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  3. H. Takano
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  4. K. Ono
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  5. S. Takio
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Corresponding author

Correspondence to S. Takio.

Additional information

Communicated by P. Ozias-Akins

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Fukuda, T., Sakai, M., Takano, H. et al. Hypermethylation of retrotransposons in the liverwort Marchantia paleacea var. diptera . Plant Cell Rep 22, 594–598 (2004). https://doi.org/10.1007/s00299-003-0739-x

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  • DOI: https://doi.org/10.1007/s00299-003-0739-x

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