Abstract
The phytohormone ethylene is involved in many developmental processes, including leaf and flower senescence. Ethylene is perceived by plants through receptors that trigger the downstream signal transduction pathway. The mutated ethylene receptor ERS1 (ethylene response sensor) from Arabidopsis is of a dominant negative nature and confers ethylene insensitivity in Arabidopsis. To investigate if the altered ERS1 gene can affect the tissue senescence in heterologous plants, we introduced it into coriander by Agrobacterium-mediated transformation. Transgenic plants were regenerated by cocultivating hypocotyl segments with A. tumefaciens harboring binary vector pCGN1547 that carried the ERS1 gene. The presence and expression of the transgene were confirmed by genomic Southern blot and reverse transcriptase-PCR analyses. Leaf and flower senescence were delayed significantly in the transgenic plants. The ability of the mutated ERS1 gene to confer the ethylene-insensitive phenotype can be exploited for extending the shelf-life of leafy vegetables.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abeles FB, Morgan PW, Saltveit ME JR (1992) Ethylene in Plant Biology. Academic, New York
Andarwulan N, Shetty K (1999) Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium-transformed roots of anise (Pimpinella anisum L.). J Agric Food Chem 47:1776–1780
Bovy AG, Angenent GC, Dons HJM, van Altvorst AC (1999) Heterologous expression of the Arabidopsis etr1-1 allele inhibits the senescence of carnation flowers. Mol Breed 5:301–308
Cahoon EB, Shanklin J, Ohlrogge JB (1992) Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco. Proc Natl Acad Sci USA 89:11184–11188
Curtis IS, Power JB, Blackhall NW, Laat AMM de, Davey MR (1994) Genotype-independent transformation of lettuce using Agrobacterium tumefaciens. J Exp Bot 45:1441–1449
Dehesh K, Jones A, Knutzon DS, Voelker TA (1996) Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB2, a thioesterase cDNA from Cuphea hookeriana. Plant J 9:167–172
Hamilton AJ, Lycett GW, Grierson D (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346:284–287
Hua J, Meyerowitz EM (1998) Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana. Cell 94:261–271
Hua J, Chang C, Sun Q, Meyerowitz EM (1995) Ethylene insensitivity conferred by Arabidopsis ERS gene. Science 269:1712–1714
Kim SW, Park MK, Liu JR (1996) High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of coriander (Coriandrum sativum L.). Plant Cell Rep 15:751–753
Lichtenthaler HK, Wellburn AR (1983) Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592
Liu Q, Singh S, Green A (2000) Genetic modification of cottonseed oil using inverted-repeat gene-silencing techniques. Biochem Soc Trans 28:927–929
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473-497
Nathan MJ, Goh CJ, Kumar PP (1992) In vitro propagation of Heliconia psittacorum by bud culture. HortScience 27:450–452
Nooden LD (1988) Whole plant senescence. In: Nooden LD, Leopold AC (eds) Senescence and aging in plants. Academic, New York, pp 391–439
Rao CD, Goh CJ, Kumar PP (1996) High frequency adventitious shoot regeneration from excised leaves of Paulownia spp. cultured in vitro. Plant Cell Rep 16:204–209
Shaw JF, Chen HH, Tsai MF, Kuo CI, Huang LC (2002) Extended flower longevity of Petunia hybrida plants transformed with boers, a mutated ERS gene of Brassica oleracea Mol Breed 9:211–216
Stepanova AN, Ecker JR (2000) Ethylene signaling: from mutants to molecules. Curr Opin Plant Biol 3:353–360
Weber N, Klein E, Mukherjee KD (1997) Metabolism of dietary petroselinic acid: a dead-end metabolite of desaturation/chain elongation reactions. Nutr Res 17:89–97
Wilkinson JQ, Lanahan MB, Clark DG, Bleecker AB, Chang C, Meyerowitz EM, Harry JK (1997) A dominant mutant receptor from Arabidopsis confers ethylene insensitivity in heterologous plants. Nat Biotechnol 15:444–447
Yang SF, Hoffman NE (1984) Ethylene bisynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189
Yoshida S, Ito M, Nishida I, Watanabe A (2001) Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana. Plant Cell Physiol 42:170–178
Yu H, Yang SH, Goh CJ (2000) DOH1, a class 1 knox gene is required for maintenance of the basic plant architecture and floral transition in orchid. Plant Cell 12:2143–2159
Acknowledgements
We thank Prof. Elliot M. Meyerowitz, California Institute of Technology, Pasadena for the gift of the Arabidopsis ERS1 clone, and Dr. Hao Yu for helpful discussions. This work was funded by a research grant (No. R-154-000-073-112) from the National University of Singapore.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by P. Lakshmanan
Rights and permissions
About this article
Cite this article
Wang, Y., Kumar, P.P. Heterologous expression of Arabidopsis ERS1 causes delayed senescence in coriander. Plant Cell Rep 22, 678–683 (2004). https://doi.org/10.1007/s00299-003-0738-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-003-0738-y