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Callus induction and regeneration in Spirodela and Lemna

  • Cell Biology and Morphogenesis
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Abstract

The development of tissue culture systems in duckweeds has, to date, been limited to species of the genus Lemna. We report here the establishment of an efficient tissue culture cycle (callus induction, callus growth and plant regeneration) for Spirodela oligorrhiza Hegelm SP, Spirodela punctata 8717 and Lemna gibba var. Hurfeish. Significant differences were found among the three duckweed species pertaining to carbohydrate and phytohormone requirements for callus induction, callus growth and frond regeneration. In vitro incubation with poorly assimilated carbohydrates such as galactose (S. oligorrhiza SP and L. gibba var. Hurfeish) and sorbitol (S. punctata 8717) as sole carbon source yielded high levels of callus induction on phytohormone-supplemented medium. Sorbitol is required for optimal callus growth of S. oligorrhiza SP and S. punctata 8717, while sucrose is required for callus growth of L. gibba var. Hurfeish. Sucrose either alone (S. oligorrhiza SP, L. gibba var. Hurfeish) or in addition to sorbitol (S. punctata 8717) is required for frond regeneration.

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Abbreviations

ABA::

(±)-Abscisic acid

BA::

N6-Benzyladenine

2,4-D :

2,4-Dichlorophenoxyacetic acid

Dicamba::

3,6-Dichloro-2-methoxybenzoic acid

2iP::

N6-(2-Isopentenyl)adenine

NAA::

α-Naphthaleneacetic acid

PCA::

p-Chlorophenoxy acetic acid

Picloram::

4-Amino-3,5,6-trichloropicolinic acid

TDZ::

Thidiazuron

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Acknowledgements

We thank D. Heller for expert assistance. The research was supported in part by Bayer CropScience AG.

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Author notes

  1. M. Jain

    Present address: Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL, 32826-3227, USA

  2. J. Vishnevetsky

    Present address: Department of Fruit Tree Sciences, Agricultural Research Organization, The Volcani Center, 50250, Bet Dagan, Israel

  3. U. Hanania

    Present address: Department of Fruit Tree Sciences, Agricultural Research Organization, The Volcani Center, 50250, Bet Dagan, Israel

Authors and Affiliations

  1. Department of Plant Sciences, Weizmann Institute of Science, 76100, Rehovot, Israel

    J. Li, M. Jain, R. Vunsh, J. Vishnevetsky, U. Hanania & M. Edelman

  2. Department of Fruit Tree Sciences, Agricultural Research Organization, The Volcani Center, 50250, Bet Dagan, Israel

    M. Flaishman & A. Perl

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  1. J. Li
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  2. M. Jain
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  8. M. Edelman
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Corresponding author

Correspondence to M. Edelman.

Additional information

Communicated by A. Altman

J. Li and M. Jain contributed equally to the research reported in this article.

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Li, J., Jain, M., Vunsh, R. et al. Callus induction and regeneration in Spirodela and Lemna . Plant Cell Rep 22, 457–464 (2004). https://doi.org/10.1007/s00299-003-0724-4

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  • DOI: https://doi.org/10.1007/s00299-003-0724-4

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