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Characterization of salicylic acid-induced genes in Chinese cabbage

  • Genetics and Genomics
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Abstract

Salicylic acid is a messenger molecule in the activation of defense responses in plants. In this study, we isolated four cDNA clones representing salicylic acid-induced genes in Chinese cabbage (Brassica rapa subsp. pekinensis) by subtractive hybridization. Of the four clones, the BC5-2 clone encodes a putative glucosyltransferase protein. The BC5-3 clone is highly similar to an Arabidopsis gene encoding a putative metal-binding farnesylated protein. The BC6-1 clone is a chitinase gene with similarities to a rapeseed class IV chitinase. Class IV chitinases have deletions in the chitin-binding and catalytic domains and the BC6-1 chitinase has an additional deletion in the catalytic domain. The BCP8-1 clone is most homologous to an Arabidopsis gene that contains a tandem array of two thiJ-like sequences. These four cabbage genes were barely expressed in healthy leaves, but were strongly induced by salicylic acid and benzothiadiazole. Expression of the three genes represented by the BC5-2, BC5-3 and BCP8-1 clones were also induced by Pseudomonas syringae pv. tomato, a nonhost pathogen that elicits a hypersensitive response in Chinese cabbage. None of these four genes, however, was strongly induced by methyl jasmonate or by ethylene.

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Abbreviations

BTH :

Benzothiadiazole

DIG :

Digoxigenin

GAPD :

Glyceraldehyde 3-phosphate dehydrogenase

HMP :

Hydroxymethylpyrimidine

HR :

Hypersensitive response

Me-JA :

Methyl jasmonate

Pst :

Pseudomonas syringae pv. tomato

SAR :

Systemic acquired resistance

References

  • Bak S, Nielsen HL, Halkier BA (1998) The presence of CYP79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates. Plant Mol Biol 38:725–734

    Article  CAS  PubMed  Google Scholar 

  • Bent AF (1996) Plant disease resistance genes: function meets structure. Plant Cell 8:1757–1771

    CAS  Google Scholar 

  • Chong J, Baltz R, Schmitt C, Beffa R, Fritig B, Saindrenan P (2002) Downregulation of a pathogen-responsive tobacco UDP-Glc:phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance. Plant Cell 14:1093–1107

    Google Scholar 

  • Dangl JL, Jones JDG (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833

    Google Scholar 

  • De Vries S, Hoge H, Bisseling T (1988) Isolation of total and polysomal RNA from plant tissues. In: Gelvin SB, Schilperoot RA (eds) Plant molecular biology. Kluwer, Dordrecht, pp B6/1–5

  • Dykema PE, Sipes PR, Marie A, Biermann BJ, Crowell DN, Randall SK (1999) A new class of proteins capable of binding transition metals. Plant Mol Biol 41:139–150

    Article  CAS  PubMed  Google Scholar 

  • Goerlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel K-H, Oostendorp M, Staub T, Ward E, Kessmann H, Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8:629–643

    CAS  PubMed  Google Scholar 

  • Halio SB, Blumenthals II, Short SA, Merrill BM, Kelly RM (1996) Sequence, expression in Escheria coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol 178:2605–2612

    CAS  PubMed  Google Scholar 

  • Hammond-Kosak KE, Jones JDG (1996) Resistance gene-dependent plant defense responses. Plant Cell 8:1773–1791

    CAS  PubMed  Google Scholar 

  • Himelblau E, Mira H, Lin SJ, Culotta VC, Penarrubia L, Amasino RM (1998) Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from Arabidopsis. Plant Physiol 117:1227–1234

    Article  CAS  PubMed  Google Scholar 

  • Jones PR, Moeller BL, Hoej PB (1999) The UDP-glucose:hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. J Biol Chem 274:35483–35491

    Article  CAS  PubMed  Google Scholar 

  • Lam E, Kato N, Lawton M (2001) Programmed cell death, mitochondria and the plant hypersensitive response. Nature 411:848–853

    Google Scholar 

  • Lamb C, Dixon RA (1997) The oxidative burst in plant disease resistance. Annu Rev Plant Physiol Plant Mol Biol 48:251–275

    CAS  Google Scholar 

  • Lange J, Mohr U, Wiemken A, Boller T, Vogeli-Lange R (1996) Proteolytic processing of class IV chitinase in the compatible interaction of bean roots with Fusarium solani. Plant Physiol 111:1135–1144

    Article  CAS  PubMed  Google Scholar 

  • Li Y, Baldauf S, Lim E-K, Bowles DJ (2001) Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana. J Biol Chem 276:4338–4343

    Article  CAS  PubMed  Google Scholar 

  • Lin S-J, Culotta VC (1995) The ATX1 gene of Saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen toxicity. Proc Natl Acad Sci USA 92:3784–3788

    CAS  PubMed  Google Scholar 

  • Martin RC, Mok MC, Mok DWS (1999) A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris. Plant Physiol 120:553–557

    Article  CAS  PubMed  Google Scholar 

  • McConn M, Creelman RA, Bell E, Mullet JE, Browse J (1997) Jasmonate is essential for insect defense in Arabidopsis. Proc Natl Acad Sci USA 94:5473–5477

    CAS  PubMed  Google Scholar 

  • McDowell JM, Dangl JL (2000) Signal transduction in the plant immune response. Trends Biochem Sci 25:79–82

    CAS  PubMed  Google Scholar 

  • Mizote T, Tsuda M, Smith DDS, Nakayama H, Nakazawa T (1999) Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase. Microbiology 145:495–501

    CAS  PubMed  Google Scholar 

  • Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SMM, Ariga H (1997) DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem Biophys Res Commun 231:509–513

    Article  CAS  PubMed  Google Scholar 

  • Nambara E, McCourt P (2000) Protein farnesylation in plants: a greasy tale. Curr Opin Plant Biol 2:388–392

    Article  Google Scholar 

  • Neuhaus J-M (1999) Plant chitinase (PR-3, PR-4, PR-8, PR-11). In: Datta SK, Muthukrishnan S (eds) Pathogenesis-related proteins in plants, CRC Press, Boca Raton, pp 77–105

  • Nuernberger T, Scheel D (2001) Signal transmission in the plant immune response. Trends Plant Sci 6:372–379

    PubMed  Google Scholar 

  • O'Donnell PJ, Calvert C, Atzorn R, Wastemack C, Leyser HMO, Bowles DJ (1996) Ethylene as a signal mediating the wound response of tomato plants. Science 274:1914–1917

    CAS  PubMed  Google Scholar 

  • Pieterse CMJ, van Loon LC (1999) Salicylic acid-independent plant defence pathways. Trends Plant Sci 4:52–58

    PubMed  Google Scholar 

  • Rad U von, Huttl R, Lottspeich F, Gierl A, Frey M (2001) Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize. Plant J 28:633–642

    Article  PubMed  Google Scholar 

  • Rasmussen U, Bojsen K, Collinge DB (1992) Cloning and characterization of a pathogen-induced chitinase in Brassica napus. Plant Mol Biol 20:277–287

    CAS  PubMed  Google Scholar 

  • Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, Hunt MD (1996) Systemic acquired resistance. Plant Cell 8:1809–1819

    CAS  Google Scholar 

  • Ryang S-H, Chung S-Y, Lee S-H, Cha J-S, Kim HY, Cho T-J (2002) Isolation of pathogen-induced Chinese cabbage genes by subtractive hybridization employing selective adaptor ligation. Biochem Biophys Res Commun 299:352–359

    Article  CAS  PubMed  Google Scholar 

  • Szerszen JB, Szczyglowski K, Bandurski RS (1994) iaglu, a gene from Zea mays involved in conjugation of growth hormone indole-3-acetic acid. Science 265:1699–1701

    CAS  PubMed  Google Scholar 

  • Takasaki T, Hatakeyama K, Suzuki G, Watanabe M, Isogai A, Hinata K (2000) The S receptor kinase determines self-incompatibility in Brassica stigma. Nature 403:913–916

    CAS  PubMed  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    PubMed  Google Scholar 

  • Vogt T, Jones P (2000) Glycosyltransferase in plant natural product synthesis: characterization of a supergene family. Trends Plant Sci 5:380–386

    Article  CAS  PubMed  Google Scholar 

  • Wagenfeld A, Gromoll J, Cooper TG (1998) Molecular cloning of rat contraception associated protein 1 (CAP1), a protein putatively involved in fertilization. Biochem Biophys Res Commun 251:545–549

    Article  CAS  PubMed  Google Scholar 

  • Zhang FL, Casey PJ (1996) Protein prenylation: molecular mechanisms and functional consequences. Annu Rev Biochem 65:241–269

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from the Technology Development Program of the Ministry of Agriculture and Forestry, Republic of Korea (grant no. 299045-3).

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Authors and Affiliations

  1. Division of Life Sciences, Chungbuk National University, 360-763, Cheongju, Korea

    Y-S. Park, H-J. Min, S-H. Ryang, K-J. Oh, H. Y. Kim & T-J. Cho

  2. Department of Agricultural Biology, Chungbuk National University, 360-763, Cheongju , Korea

    J-S. Cha

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  1. Y-S. Park
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  2. H-J. Min
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  3. S-H. Ryang
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  4. K-J. Oh
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  7. T-J. Cho
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Correspondence to T-J. Cho.

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Communicated by I.S. Chung

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Park, YS., Min, HJ., Ryang, SH. et al. Characterization of salicylic acid-induced genes in Chinese cabbage. Plant Cell Rep 21, 1027–1034 (2003). https://doi.org/10.1007/s00299-003-0606-9

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