Abstract
A rapid and efficient micropropagation method has been established for six European poplar cultivars of economic interest – four Populus × interamericana and two Populus × euramericana. Using a three-step procedure, we were able to regenerate plantlets from callus and acclimate them within 4 months. In the first step, callogenesis was induced when explants were cultured for 25 days on culture medium supplemented with 10 µM α-naphthaleneacetic acid and 5 µM N6(2-isopentenyl)adenine. Bud regeneration followed by shoot elongation was then obtained from callus tissue by combining the cytokinin-like compound thidiazuron with the surfactant Pluronic F-68 at concentrations adjusted for each cultivar. The usefulness of this procedure in the area of genetic engineering is discussed.
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Noël, N., Leplé, JC. & Pilate, G. Optimization of in vitro micropropagation and regeneration for Populus × interamericana and Populus × euramericana hybrids (P. deltoides, P. trichocarpa, and P. nigra). Plant Cell Rep 20, 1150–1155 (2002). https://doi.org/10.1007/s00299-002-0465-9
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DOI: https://doi.org/10.1007/s00299-002-0465-9