Abstract
Agrobacterium-mediated transformation is highly dependent upon competency of the target plant tissues. It is important to develop the capacity of transformed cells to include cell proliferation and differentiation. A system which results in cell proliferation and differentiation only of transformed cells is highly desirable for plant transformation. We report here a new GST-MAT vector system (MATIMH), in which the ipt gene combined with iaaM/H genes was used as the selectable marker gene and the GST-II promoter was used as the promoter of the R gene in a site-specific recombination system. In tobacco transformation, the combination of the ipt gene and the iaaM/H genes can result in the production of both auxin and cytokinin in transformed tissues and induce regeneration of transgenic shoots exhibiting an ipt-shooty phenotype more efficiently than the ipt gene alone. When we transformed 20 tobacco leaf discs with the MATIMH vector, marker-free transgenic plants were produced from five (41.6%) out of 12 ipt-shooty lines. These results indicated that the combination of the iaaM/H genes and the ipt gene can more efficiently produce both transgenic plants and marker-free transgenic plants.
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Endo, S., Kasahara, T., Sugita, K. et al. A new GST-MAT vector containing both ipt and iaaM/H genes can produce marker-free transgenic tobacco plants with high frequency. Plant Cell Rep 20, 923–928 (2002). https://doi.org/10.1007/s00299-001-0418-8
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DOI: https://doi.org/10.1007/s00299-001-0418-8