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Cartilage collagen type II seromarker patterns in axial spondyloarthritis and psoriatic arthritis: associations with disease activity, smoking and HLA-B27

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Abstract

The aim of the study was to assess the possible association between type II collagen turnover seromarkers and disease profile in patients with axial spondyloarthritis (SpA) and psoriatic arthritis (PsA). Outpatients with axial SpA (n = 110) or PsA (n = 101) underwent clinical examination including disease activity measures and HLA-B27 typing. The procollagen IIA N-terminal peptide (PIIANP) and a matrix metalloproteinase-generated type II collagen fragment (C2M) were quantified in serum by ELISA. C2M was higher in SpA than in controls, 0.41 versus 0.36 ng/ml (p = 0.004), while PIIANP did not differ between patients and healthy subjects, 2252 versus 2142 ng/ml (p = 0.13). However, DMARD-naïve SpA patients had higher PIIANP, 2461 ng/ml (p = 0.01) and C2M, 0.44 ng/ml (p = 0.0007) levels than controls, and PIIANP correlated with CRP (ρ = 0.34). C2M was lower in SpA smokers, 0.36 ng/ml versus non-smokers, 0.43 ng/ml (p = 0.02), while PIIANP was higher in HLA-B27 positive, 2312 ng/ml versus negative patients, 2021 ng/ml (p = 0.03). In PsA, PIIANP and C2M did not differ between patients and controls, but PIIANP was elevated in patients not receiving DMARDs, 2726 ng/ml. In PsA, PIIANP and C2M did not differ according to smoking and HLA-B27. Cartilage degradation assessed by C2M is increased in SpA irrespective of treatment but not in PsA. Cartilage synthesis reflected by PIIANP is increased in untreated SpA and PsA. PIIANP correlates with CRP in SpA while not in PsA. In DMARD-naïve SpA but not in PsA, HLA-B27 positivity and smoking are associated with a chondro-proliferative metabolic pattern.

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Acknowledgments

We appreciate the expert technical assistance by Kirsten Junker.

Authors’ contributions

All authors have made substantial contributions to (1) the conception and design of the study, or acquisition of data, or analysis and interpretation of data, (2) drafting the article or revising it critically for important intellectual content, (3) final approval of the version to be submitted. Specific contributions are: (1) The conception and design of the study: HM, PJ, (2) Acquisition of data: HM, LE, AL, NG, (3) Analysis and interpretation of data: HM, NG, AC, AB, AS, GLS, PJ, (4) Drafting the article: HM, PJ, (5) Revising the article critically for important intellectual content: HM, NG, AC, LE, GLS, AL, AB, AS, PJ, (6) Final approval of the version submitted: HM, NG, AC, LE, GLS, AL, AB, AS, PJ, (7) Statistical expertise: AC, HM, PJ, (8) Obtaining of funding: HM, PJ, AB, (9) Collection and assembly of data: HM. HM takes responsibility for the integrity of the work as a whole, from inception to the final version of the article. HM was involved in the conception and design of the study, acquisition of data, analysis and interpretation of the data, drafting of the article, critical revision of the article for important intellectual content, final approval of the article, statistical expertise, obtaining of funding, and collection and assembly of data.

Funding

The current study was supported by research grants from the region of Southern Denmark and Department of Rheumatology C, Odense University Hospital, Odense, Denmark.

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Authors and Affiliations

  1. Department of Rheumatology, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense C, Denmark

    Heidi Lausten Munk & Peter Junker

  2. Rheumatology Biomarkers and Research, Nordic Bioscience, Herlev, Denmark

    Natasja Staehr Gudmann, Anne C. Bay-Jensen & Anne Sofie Siebuhr

  3. Department of Rheumatology, Vejle Hospital, Vejle, Denmark

    Anne Friesgaard Christensen & Anne Gitte Loft

  4. Department of Rheumatology, Esbjerg Hospital, Esbjerg, Denmark

    Leif Ejstrup

  5. Institute of Molecular Medicine, University of Southern Denmark, Odense C, Denmark

    Grith Lykke Sorensen

  6. Department of Rheumatology, Aarhus University Hospital, Aarhus, Denmark

    Anne Gitte Loft

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  1. Heidi Lausten Munk
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Correspondence to Heidi Lausten Munk.

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Natasja Staehr Gudmann, Anne C. Bay-Jensen and Anne Sofie Siebuhr were employed at Nordic Bioscience while this study was conducted. Anne C. bay-Jensen holds stocks in Nordic Bioscience. The authors have no financial and personal relationships with other people or organizations that could potentially and inappropriately influence (bias) their work and conclusions.

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Munk, H.L., Gudmann, N.S., Christensen, A.F. et al. Cartilage collagen type II seromarker patterns in axial spondyloarthritis and psoriatic arthritis: associations with disease activity, smoking and HLA-B27. Rheumatol Int 36, 541–549 (2016). https://doi.org/10.1007/s00296-015-3397-8

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