Abstract
Systemic lupus erythematosus (SLE) is the prototype of complex autoimmune diseases characterized by the production of autoantibodies which results in widespread immunologic abnormalities and immune complex formation. The underlying etiology remains largely unknown. When progressing toward kidney failure, it is becoming a serious public health problem. Kidney transplantation is a feasible therapy, but significant limitations were existed, including shortage of donor organs and lack of funding. To find an alternative proposal for kidney replacement, the induced pluripotent stem cells (iPSCs) technology was adopted. We identified typical SLE patients. Lentiviral transduction of OCT4, SOX2, KLF4, and c-MYC, under feeder conditions, resulted in reprogramming of urine-derived renal tubular cells. We investigated the viability of iPSCs generation from patients with SLE by identification of totipotency and pluripotency. SLE patient renal tubular cells–derived iPSCs exhibited properties of human embryonic stem cells, including morphology, growth properties, alkaline phosphatase, expression of pluripotency, genes and surface markers, and teratoma formation. We demonstrated that generation of SLE-specific iPSCs from urine was not only the first time worldwide, but was feasible and efficient. IPSCs from SLE would provide convenient model to study disease pathogenesis, drugs screening, and gene therapy.
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Acknowledgments
This work was supported by grants from the Key Project for Science and Technology of Shenzhen (project no. 201001006) and the General Program of National Natural Science Foundation China (30972741/C080701). This work was supported by the Guangzhou Institutes of Biomedicine and Health (in Guangzhou, China). We thank all members of laboratories for helpful suggestions, especially Dr. Qin Dajiang, and all the patients and healthy volunteers who participated in this study.
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The authors declare that they have no conflict of interest.
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Chen, Y., Luo, R., Xu, Y. et al. Generation of systemic lupus erythematosus-specific induced pluripotent stem cells from urine. Rheumatol Int 33, 2127–2134 (2013). https://doi.org/10.1007/s00296-013-2704-5
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DOI: https://doi.org/10.1007/s00296-013-2704-5