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Phosphorylation of osteopontin in osteoarthritis degenerative cartilage and its effect on matrix metalloprotease 13

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Abstract

The purpose of this study is to observe the differences of osteopontin (OPN) phosphorylation in osteoarthritis (OA) cartilage and normal cartilage, and evaluate the possible correlations between the OPN phosphorylation and MMP-13 expression. Degenerative cartilage (n = 29) and normal cartilage (n = 10) were identified by hematoxylin-eosin, safranin-O staining and modified Mankin score. The phosphorylation level of OPN in OA cartilage and normal cartilage was detected by immunoprecipitation. Chondrocytes were treated with phospho-OPN, OPN or buffer. Quantitative reverse transcription polymerase chain reaction (qPCR) and ELISA were used to assess the expression of MMP-13 in different treatments. The OD values of phosphorylation of OPN in normal cartilage and OA cartilage were 137.89 ± 10.59 and 153.52 ± 8.80, respectively, (P = 0.000). Chondrocytes treated with OPN showed a higher MMP-13 expression at gene and protein level compared with control group. Chondrocytes treated with phospho-OPN showed the highest MMP-13 expression in gene and protein. In conclusion, our results revealed a higher phosphorylation level of OPN in OA cartilage than in normal cartilage. We found OPN leads to elevated expression of MMP-13 (both at gene level and protein level), and phospho-OPN had a more obvious upregulation effect on MMP-13 expression than nonphospho-OPN. Further studies are needed to reveal the mechanism of OPN phosphorylation on cartilage degeneration.

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Acknowledgments

We thank the reviewers and the Rheumatology International (Clinical and Experimental Investigations) manuscript editorial staffs for their helpful comments that have helped to considerably improve this paper. Mai Xu has received Sports Medicine Research Fund of Central South University. Shuguang Gao is currently receiving the Young Teacher’s boosting project of the Fundamental Research Funds for the Central Universities in Central South University, National Natural Science Foundation of China (81201420). Guanghua Lei has received the National 863 project of China (2011AA030101), National Natural Science Foundation of China (81272034), the Provincial Science Foundation of Hunan, the freedom explore Program of Central South University (2012QNZT103), Distinguished Young Scientists fund of Central South university, the foundation of development and reform commission of Hunan province and National Clinical Key Department Construction Projects of China. For the remaining authors none were declared.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopaedics, Xiangya Hospital, Central South University, No. 87 Xiangya Road, 410008, Changsha, Hunan, China

    Mai Xu, Shuguang Gao & Guanghua Lei

  2. Center for Molecular Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, 410008, Changsha, Hunan, China

    Lu Zhang

  3. Institutes of Biology and Medical Sciences, Suzhou University, No. 199 Renai Road, 215123, Suzhou, Jiangsu, China

    Lei Zhao

  4. Department of Orthopaedics, Qingdao Haici Hospital, No. 4 Renmin Road, 266000, Qingdao, Shandong, China

    Rui Han

  5. Department of Orthopaedics, Zhuzhou No.1 Hospital, No.23 Station Road, 412000, Zhuzhou, Hunan, China

    Dazhi Su

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  1. Mai Xu
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Correspondence to Guanghua Lei.

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Xu, M., Zhang, L., Zhao, L. et al. Phosphorylation of osteopontin in osteoarthritis degenerative cartilage and its effect on matrix metalloprotease 13. Rheumatol Int 33, 1313–1319 (2013). https://doi.org/10.1007/s00296-012-2548-4

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