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HLA-B27 subtypes in Turkish patients with ankylosing spondylitis and healthy controls

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Abstract

The aim of this study was to determine human leukocyte antigen (HLA)-B27 subtypes frequency in ankylosing spondylitis (AS) and related spondyloartropathy (SpA) patients. Therefore, we investigated the differences in HLA-B27 subtypes between HLA-B27-positive patients and controls. Sixty six patients were included in this study (51 AS and 15 SpA). Thirty-five individuals were diagnosed with leukemia or chronic renal failure, and their donors without any rheumatological problem (no SpA history) were selected as the control group. HLA-B27 subtyping was performed by PCR-SSP (polymerase chain reaction with sequence-specific primer) method in serologically HLA-B27-positive 46 AS patients, 9 SpA patients and control group. When the frequency of HLA-B27 was 4.5% in Turkish population, this frequency was 90.2% in AS patients. Four different HLA-B27 subtypes found in AS patients were B*2705 (65.2%), B*2702 (26.1%), B*2704 (6.5%) and B*2707 (2.2%). In SpA patients, B*2705 and B*2702 found in equal frequency. Five B27 alleles were identified in our control group: B*2705 (54.3%), B*2702 (31.4) %, B*2703 (2.9%), B*2704 (2.9%) and B*2702/B*2705 (8.5%). Both in the patient group and in the control group, we also observed B*2705 as most frequent allele, and B*2702 was second common allele. Our results show that the frequency of HLA-B27 subtypes is not significantly different between patients and controls (P > 0.10).

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Authors and Affiliations

  1. Department of Genetics, Selcuk University Medical School, Konya, Turkey

    Muradiye Acar, Tulin Cora & Hasan Acar

  2. Department of Rheumatology, Selcuk University Medical School, Konya, Turkey

    Recep Tunc

Authors
  1. Muradiye Acar
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  2. Tulin Cora
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  3. Recep Tunc
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  4. Hasan Acar
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Correspondence to Muradiye Acar.

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Acar, M., Cora, T., Tunc, R. et al. HLA-B27 subtypes in Turkish patients with ankylosing spondylitis and healthy controls. Rheumatol Int 32, 3103–3105 (2012). https://doi.org/10.1007/s00296-011-2099-0

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  • DOI: https://doi.org/10.1007/s00296-011-2099-0

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