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Connective tissue growth factor, a regulator related with 10-hydroxy-2-decenoic acid down-regulate MMPs in rheumatoid arthritis

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Abstract

10-hydroxy-2-decenoic acid (10H2DA) is suggested to be a potential medication for rheumatoid arthritis (RA) by activation of matrix metalloproteinases (MMPs) via mitogen-activated protein kinase signaling pathways. The aim of the present work was to seek differentially expressed proteins in rheumatoid arthritis synovial fibroblasts (RASFs) treated with 10H2DA by comparative proteomics analysis. Two-dimensional electrophoresis (2-DE) and LC–MS/MS were performed to identify changes in protein expression after 24-h 10H2DA treatment. Differentially expressed proteins were identified by real-time PCR and Western blot analysis. Influence of down-regulation of connective tissue growth factor (CTGF) expression on MMPs was studied by RNAi. Ten proteins were up-regulated and 9 proteins were down-regulated after 24-h 10H2DA treatment. A total of 19 differentially expressed proteins were identified and found to be associated with glycolysis, lipid metabolism, cell adhesion, ATP synthesis, oxidation reduction, and anti-apoptosis. CTGF, a member of the C-terminal cystein-rich proteins (CCN) family, was down-regulated after 24-h 10H2DA treatment. MMPs were down-regulated after RNAi (CTGFi). These results suggest that CTGF is a regulator factor in the expression of MMPs, and 10H2DA down-regulate the concentration of MMPs probably by down-regulating the expression of CTGF.

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Abbreviations

CTGF:

Connective tissue growth factor

10H2DA:

10-Hydroxy-2-decenoic acid

RASFs:

Rheumatoid arthritis synovial fibroblasts

2-DE:

Two-dimensional electrophoresis

RA:

Rheumatoid arthritis

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Acknowledgments

We are grateful to Ming Li for technical assistance. This work was partially supported by the grant Y2006A021 from Wenzhou Science Technology Bureau and 2010R413018 from Zhejiang college students’ innovative research team.

Conflict of interest

The authors have declared that no conflict of interest exists.

Author information

Authors and Affiliations

  1. Department of Medicinal Chemistry, Pharmacy School, Wenzhou Medical College, 3#1301 Nanou Jingyuan, Shengxin Road, Wenzhou, 325000, China

    Xin-yu Yang

  2. Department of Biochemistry School of Basic Medical Sciences, Wenzhou Medical College, Wenzhou, China

    Jian-guang Wang, Jian Ruan, Chun-yang Li, Wei Zhang, Hui Ye, Nian-han Shen, Kang-fu Lei & Xiu-fang Chen

  3. Department of Orthopaedic Surgery, Shanghai Guanghua Special Hospital for Rheumatoid Arthritis, Shanghai, China

    Wei-tao Zhai

  4. Department of Rheumatology, Jiamusi Central Hospital, Jiamusi, China

    Jian-min Wang

  5. Department of Emergency, First People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China

    Yan Li

Authors
  1. Jian-guang Wang
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  2. Jian Ruan
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  3. Chun-yang Li
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  4. Jian-min Wang
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  5. Yan Li
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  10. Kang-fu Lei
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  11. Xiu-fang Chen
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  12. Xin-yu Yang
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Corresponding authors

Correspondence to Yan Li or Xin-yu Yang.

Additional information

Jian-guang Wang and Jian Ruan contributed equally to this study.

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Wang, Jg., Ruan, J., Li, Cy. et al. Connective tissue growth factor, a regulator related with 10-hydroxy-2-decenoic acid down-regulate MMPs in rheumatoid arthritis. Rheumatol Int 32, 2791–2799 (2012). https://doi.org/10.1007/s00296-011-1960-5

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  • DOI: https://doi.org/10.1007/s00296-011-1960-5

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