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Lipoic acid prevents steroid-induced osteonecrosis in rabbits

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Abstract

The objective of this study was to investigate in vivo effects of lipoic acid (LA) in preventing steroid-induced osteonecrosis and the possible pathway in a rabbit model. Sixty rabbits were divided into 2 groups: rabbits were intraperitoneally injected with LA aqueous solution at 36 mg/kg of body weight per day for 4 weeks in Group A and rabbits were injected with physiologic saline (PS) as a control in Group B. At 2 weeks after starting treatment, they were intramuscularly injected once with 20 mg/kg of methylprednisolone acetate (MPSL). The femora were histopathologically examined for the presence of osteonecrosis. The plasma levels of total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), glutathione (GSH), endothelin (ET) and malondialdehyde (MDA) were assayed at 2 weeks after the injection of MPSL. The incidence of osteonecrosis was significantly higher in Group B (73.1%) than in Group A (20.8%). The GSH level was higher in Group A than in Group B after the LA injection. The plasma MDA and ET levels were lower in Group A than in Group B at 2 weeks after the MPSL administration. Lipoic acid can prevent the development of steroid-induced osteonecrosis in rabbits. Inhibited oxidative stress and amendment of vascular endothelial dysfunction is a possible mechanism for this effect.

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Acknowledgments

The authors wish to thank zhi jie Zhang, MD for the technical support. We also thank Jian- Zhong Hu, MD and Guang-Hua Lei, MD for their contributions to this study.

Conflict of interest

The authors have declared no conflicts of interest.

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Authors and Affiliations

  1. Department of Orthopedics, Xiangya Hospital, Central-South University, Xiangya Road 87#, Kaifu, Changsha, Hunan, 410008, People’s Republic of China

    Bang-Bao Lu & Kang-Hua Li

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  1. Bang-Bao Lu
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  2. Kang-Hua Li
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Correspondence to Kang-Hua Li.

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Lu, BB., Li, KH. Lipoic acid prevents steroid-induced osteonecrosis in rabbits. Rheumatol Int 32, 1679–1683 (2012). https://doi.org/10.1007/s00296-011-1846-6

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  • DOI: https://doi.org/10.1007/s00296-011-1846-6

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