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Melatonin attenuates clock gene Cryptochrome1, which may aggravates mouse anti-type II collagen antibody-induced arthritis

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Abstract

Very recently, the circadian rhythm was proved to play an important role in the pathogenesis of arthritis. The role of melatonin in the development and progress of rheumatoid arthritis has been implicated for decades. This study was aimed to investigate the effect of melatonin on the expression of circadian clock genes in mouse anti-type II collagen antibody-induced arthritis (CIA). Mice were divided into 3 groups: control, CIA, and CIA + melatonin treatment (MLT). Both mRNA and protein levels of circadian clock gene Cryptochrome1 (Cry1) were markedly decreased in CIA + MEL group compared with those in control and CIA groups. MLT increased paw thickness. Histologic and X-ray assessment also revealed increased infiltration of inflammatory cells, synovial hyperplasia, and the destruction of articular cartilage and bone by MLT. The concentrations of anti-type II collagen antibody in CIA + MEL group mice were significantly higher than those in control and CIA groups (P < 0.05). Serum concentrations of TNF-α (P < 0.005) and IL-6 (P < 0.05) in CIA + MLT group were also increased. Taken together, these results implicate that clock gene Cry1 may be involved in the aggravation of MLT-mediated arthritis in mice anti-type II collagen antibody-induced arthritis.

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Acknowledgments

This work was supported by the research promoting grant from the Keimyung University Dongsan Medical Center in 2009.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea

    Jihye Bang & Eunyoung Ha

  2. Department of Radiology, School of Medicine, Keimyung University, Daegu, Republic of Korea

    Hyuk Won Chang

  3. Department of Pathology, School of Medicine, Keimyung University, Daegu, Republic of Korea

    Hae-Ra Jung

  4. Department of Orthopedic Surgery, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea

    Chul-Hyun Cho

  5. Department of Internal Medicine, School of Medicine, Yeungnam University, Daegu, Republic of Korea

    Ji-An Hur

  6. Department of Rheumatology, College of Medicine and Institute of Health Science, Gyeongsang National University, Jinju, Republic of Korea

    Sang-Il Lee

  7. Department of Plastic and Reconstructive Surgery, Seoul National University, College of Medicine, Seoul, Republic of Korea

    Tae Hyun Choi

  8. Department of Rheumatology, Pain Research Center, School of Medicine, Institute for Medical Science, Keimyung University, Daegu, Republic of Korea

    Sang-Hyon Kim

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  1. Jihye Bang
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  9. Eunyoung Ha
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Correspondence to Sang-Hyon Kim or Eunyoung Ha.

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Bang, J., Chang, H.W., Jung, HR. et al. Melatonin attenuates clock gene Cryptochrome1, which may aggravates mouse anti-type II collagen antibody-induced arthritis. Rheumatol Int 32, 379–385 (2012). https://doi.org/10.1007/s00296-010-1641-9

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  • DOI: https://doi.org/10.1007/s00296-010-1641-9

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