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Comparative proteome analysis of peripheral blood mononuclear cells in systemic lupus erythematosus with iTRAQ quantitative proteomics

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Abstract

To identify and quantify protein profiles from peripheral blood mononuclear cells (PBMC) of systemic lupus erythematosus (SLE) patients with isobaric Tagging for Relative and Absolute protein Quantification (iTRAQ)–based proteomic technology and to find differentially expressed proteins in SLE. PBMC were collected from patients of six stable SLE, six active SLE, six rheumatoid arthritis (RA), and six healthy donors. After protein extraction and concentration, the pooled protein content was labeled with iTRAQ reagents and then subjected to multiple chromatographic fractionation and tandem mass spectrometry. ProteinPilot™ 3.0 software and a database of IPI (International Protein Index) human 3.62 were used for database searching and statistical analysis. A total of 452 proteins were identified. Of these, 67 unique proteins were observed twofold or more alteration in levels across groups. The proteins determined support existing knowledge and uncover novel biomarker candidates. These results indicate that iTRAQ-based technology can serve as a useful aid for identification and quantification proteins from PBMC.

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Acknowledgments

We thank the patients and healthy volunteers who participated in this study and Shuiming Li for his technical assistance. This work was supported by grants from the Key Project for Science and Technology of Shenzhen (project no. 201001001).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, 1 Yi Xue Yuan Road, Chongqing, 400016, People’s Republic of China

    Linqian Wang, Suwen Qi & Zhiguang Tu

  2. The Second Clinical Medical College, Jinan University, Shenzhen People’s Hospital, Shenzhen, Guangdong Province, People’s Republic of China

    Yong Dai, Baodong Sun, Jinli Wen & Li Zhang

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  1. Linqian Wang
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  2. Yong Dai
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  5. Jinli Wen
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  6. Li Zhang
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  7. Zhiguang Tu
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Correspondence to Zhiguang Tu.

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Wang, L., Dai, Y., Qi, S. et al. Comparative proteome analysis of peripheral blood mononuclear cells in systemic lupus erythematosus with iTRAQ quantitative proteomics. Rheumatol Int 32, 585–593 (2012). https://doi.org/10.1007/s00296-010-1625-9

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  • DOI: https://doi.org/10.1007/s00296-010-1625-9

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