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Evaluation of inflammatory change and bone erosion using a murine type II collagen-induced arthritis model

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Abstract

The exact mechanism of rheumatoid arthritis (RA) is unclear, but a combination of genetic, environmental and hormonal factors is thought to be involved. This study examined the progressive arthritic reaction of murine type II collagen-induced arthritis (CIA), a representative animal model of RA. Arthritic reactions, including inflammation and bone erosion were examined using an objective non-invasive method. Two scoring systems were used to evaluate changes in cutaneous inflammation and bone erosion during RA progression. The severity of inflammation was evaluated by visual scoring of erythema and edema, while the degree of bone erosion was quantified by macroradiographical erosion analysis of specific bones. A significant difference was observed in both visual (P = 0.0001, n = 7) and radiographic (P < 0.0001, n = 7) examinations for the RA group as compared to the control. The relationship between inflammatory change and erosive change in bone showed a significant positive correlation, r = 0.9550 (P < 0.0001, n = 7). The overall rate of asymmetry was 25.23% in both fore- and hindpaws. The results generated from these experiments show that murine CIA is a promising model for elucidating the mechanism of RA. In addition, the results of this study may be used for monitoring RA progression as well as screening therapy efficacy in the joint pathology.

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Abbreviations

ANOVA:

Analysis of variance

AP:

Anterior–posterior

CFA:

Complete Freund adjuvant

CIA:

Collagen-induced arthritis

IFA:

Incomplete Freund adjuvant

MCP:

Metacarpophalangeal

MTP:

Metatarsophalangeal

PA:

Posterior–anterior

PIP:

Proximal interphalangeal

r :

Pearson correlation coefficient

RA:

Rheumatoid arthritis

ROI:

Region of interest

SD:

Standard deviation

TNF:

Tumor necrosis factor

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Acknowledgments

This study was supported by a research fund from Seoul R&BD (Grant # CR070054). The authors would like to express deep gratitude for the support.

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Authors and Affiliations

  1. Department of Biomedical Engineering, College of Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea

    Samjin Choi, Gi-Ja Lee, Sung Wook Kang, Ji-Hye Park, Jeong-Hoon Park & Hun-Kuk Park

  2. Healthcare Industry Research Institute, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Samjin Choi, Gi-Ja Lee, Ji-Hye Park, Jeong-Hoon Park & Hun-Kuk Park

  3. Division of Rheumatology, Department of Internal Medicine, College of Medicine, Kyung Hee University Medical Center, Seoul, 130-702, Republic of Korea

    Yeon-Ah Lee & Seung-Jae Hong

  4. Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Sung Wook Kang

  5. Program of Medical Engineering, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Hun-Kuk Park

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  1. Samjin Choi
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Correspondence to Hun-Kuk Park.

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Choi, S., Lee, YA., Hong, SJ. et al. Evaluation of inflammatory change and bone erosion using a murine type II collagen-induced arthritis model. Rheumatol Int 31, 595–603 (2011). https://doi.org/10.1007/s00296-009-1333-5

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  • DOI: https://doi.org/10.1007/s00296-009-1333-5

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