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The expression of PADI4 in synovium of rheumatoid arthritis

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Abstract

PADI4 that catalyzes the conversion of peptidylarginine to citrulline is associated with rheumatoid arthritis in some populations. The current study investigated the expressions of PADI4 in synovial fluid of RA (n = 73), osteoarthritis (OA, n = 96) and ankylosing spondylitis (AS, n = 32) using ELISA and western blotting following immuno-precipitation (n = 6 for each diseases). The study also compared the mRNA level of PADI4 in the synovial membrane of RA with the levels in the samples of OA and AS (n = 6 for each diseases) using real time PCR. ELISA detected a higher level of PADI4 in SF of RA than in samples of OA and AS (P = 0.0001). The level of PADI4 was significantly correlated with the level of rheumatic factor (P = 0.015), but not with anti cyclic citrullinated peptide antibody (anti-CCP) in the RA fluids. Western blotting confirmed the expression of PADI4 in SF of RA. Quantitative PCR measured higher transcription of PADI4 in the synovial membrane of RA than in the samples of OA and AS. The results confirmed increased expression of PADI4 in synovium of RA.

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Acknowledgments

The present study was supported by the National Natural Science Foundation of China (NTFC) (30671949), the Shandong Science and Technology Research Program (2007GG20002001, 2006GG2202050), the Shandong Scientific Instrument Equipment Promotion Transformation Project (2006GG1108097-41, 2007GG2TC02050) and the Leading Project of Medical Science of Shandong.

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Authors and Affiliations

  1. Key Laboratory for Bio-drugs of Ministry of Health, Research Center for Medicinal Biotechnology, Shandong Academy of Medical Sciences, Jingshi Road 89, 250062, Jinan, Shandong, People’s Republic of China

    Xiaotian Chang, Yan Zhao & Youming Zhu

  2. Department of Arthrosurgery, Provincial Hospital of Shandong, Jinan, Shandong, People’s Republic of China

    Shui Sun

  3. Department of Rheumatology, Shandong Hospital of Labor Health and Occupational Disease Control, Jinan, Shandong, People’s Republic of China

    Yunzhong Zhang

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  1. Xiaotian Chang
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  2. Yan Zhao
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  3. Shui Sun
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  4. Yunzhong Zhang
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  5. Youming Zhu
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Correspondence to Xiaotian Chang.

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Chang, X., Zhao, Y., Sun, S. et al. The expression of PADI4 in synovium of rheumatoid arthritis. Rheumatol Int 29, 1411–1416 (2009). https://doi.org/10.1007/s00296-009-0870-2

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