Skip to main content

Advertisement

SpringerLink
Log in

Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet’s disease etiopathogenesis

  • Original Article
  • Published:
Rheumatology International Aims and scope Submit manuscript

Abstract

The objective of this study was to investigate the expressions of toll-like receptors on neutrophils and monocytes in Behcet’s disease (BD). Forty-two patients with BD were included in the study. Baseline and stimulated cells with heat shock protein-60 (HSP-60), lipopolysaccharide (LPS) and crude extract of Streptococcus sanguis (SS) were analyzed for toll-like receptor-1, -2, -4 and -6 expressions using flow cytometer. Results were confirmed using semi-quantitative PCR technique. The mean frequency of TLR-6 expressing granulocytes in BD patients was significantly lower than in controls (4.2 ± 0.6 vs. 9.2 ± 0.6 and 8.0 ± 1.4, BD vs. rheumatoid arthritis (RA) and healthy control (HC); P < 0.05). TLR-6 expressing granulocyte population was enhanced after stimulation with HSP-60 and SS (20 ± 0.5 and 12.8 ± 0.6, respectively). Decreased TLR-2 expression was noted in monocytes of BD patients after stimulation with HSP-60 and LPS (71.0 ± 0.5 and 73.0 ± 0.5; P < 0.02, for both antigens). Functional abnormalities of these receptors or different activation cascades by different microorganisms are associated with disease pathogenesis in Behcet’s disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Yazici H, Yurdakul S, Hamuryudan V (1998) Behcet’s syndrome. In: Klippel JH, Dieppe PA (eds) Rheumatology. Mosby, London, 7:26:1–6

  2. Mumcu G, Inanc N, Yavuz S, Direskeneli H (2007). The role of infectious agents in the pathogenesis, clinical manifestations and treatment strategies in Behcet’s disease. Clin Exp Rheumatol, Sept. 2007 Supplement

  3. Lehner T, Lavery E, Smith R et al (1991) Association between the 65 kDa heat shock protein, Streptoccocus sanguis and the corresponding antibodies in Behcet’s syndrome. Infect Immun 59:1434–1441

    PubMed  CAS  Google Scholar 

  4. Mumcu G, Ergun T, Inanc N et al (2004) Oral health is impaired in Behcet’s disease and associated with disease severity. Rheumatology 43:1028–1033

    Article  PubMed  CAS  Google Scholar 

  5. Direskeneli H, Eksioglu-Demiralp E, Yavuz S, Ergun T, Lehner T, Akoglu T (2000) T cell responses to 60/65 kDa HSP derived peptides in Turkish Behcet’s disease patients. J Rheum 27:708–713

    PubMed  CAS  Google Scholar 

  6. Miura T, Ishihara K, Kato T et al (2005) Detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with Behcet’s disease. Oral Microbial Immunol 20:167–171

    Article  CAS  Google Scholar 

  7. Kol A, Lichtman AH, Finberg RW, Libby P, Kurt-Jones EA (2000) Heat shock protein 60 activates the innate immune response: CD14 is an essential receptor for HSP-60 activation of mononuclear cells. J Immunol 164:13–17

    PubMed  CAS  Google Scholar 

  8. Chen W, Syldath U, Bellman K, Burkart V, Kolb H (1999) Human 60-kDa Heat shock protein: a danger signal to the innate immune system. J Immunol 162:3212–3219

    PubMed  CAS  Google Scholar 

  9. Takeda K, Kaisho T, Akira S (2003) Toll-like receptors. Annu Rev Immunol 21:335–376

    Article  PubMed  CAS  Google Scholar 

  10. Henneke P, Morath S, Uematsu S et al (2005) Role of lipoteichoic acid in the phagocyte response to group B streptococcus. J Immunol 174:6449–6455

    PubMed  CAS  Google Scholar 

  11. Takeuchi O, Kawai T, Muhiracht PF et al (2001) Discrimination of bacterial lipoproteins by toll-like receptor 6. Int Immunol 13:933–940

    Article  PubMed  CAS  Google Scholar 

  12. International study group for Behcet’s disease (1990) Criteria for diagnosis of Behcet’s disease. Lancet 335:1078–1080

    Google Scholar 

  13. Nakao Y, Funami K, Kikkawa S et al (2005) Surface-expressed TLR-6 participates in the recognition of diacylated lipopeptide and peptidolgycan in human cells. J Immunol 174:1566–1573

    PubMed  CAS  Google Scholar 

  14. Omueti KO, Beyer JM, Johnson CN, Lyle EA, Tapping RI (2005) Domain exchange between human toll-like receptors 1and 6 reveals a region required for lipopeptide discrimination. J Biol Chem 280:36616–36625

    Article  PubMed  CAS  Google Scholar 

  15. Iwahashi M, Yamamura M, Aita T et al (2004) Expression of toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis. Arthritis Rheum 50:1457–1467

    Article  PubMed  CAS  Google Scholar 

  16. Kawanaka N, Yamamura M, Aita T et al (2002) CD14 + CD16 + blood monocytes and joint inflammation in rheumatoid arthritis. Arthritis Rheum 46:2578–2586

    Article  PubMed  CAS  Google Scholar 

  17. Belge KU, Dayyani F, Horelt A et al (2002) The proinflammatory CD14 + CD16 + DR++ monocytes are a major source of TNF. J Immunol 168:3536–3542

    PubMed  CAS  Google Scholar 

  18. Sahin S, Lawrence R, Direskeneli H, Hamuryudan V, Yazici H, Akoglu T (1996) Monocyte activity in Behcet’s disease. Br J Rheum 35:424–429

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Rheumatology, University of Marmara, Istanbul, Turkey

    Sule Yavuz & Haner Direskeneli

  2. Department of Haematology–Immunology, University of Marmara, Istanbul, Turkey

    Yesim Elbir, Aysin Tulunay & Emel Eksioglu-Demiralp

  3. Sedef Cad. Ata2-5 blok D:145 Atasehir, Istanbul, Turkey

    Sule Yavuz

Authors
  1. Sule Yavuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yesim Elbir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aysin Tulunay
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Emel Eksioglu-Demiralp
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Haner Direskeneli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sule Yavuz.

Additional information

Dr. Sule Yavuz’s work was supported by Turkish Academy of Sciences, in the framework of the young Scientist Award Program (EA-TUBA-GEBIP/2002-1/21).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yavuz, S., Elbir, Y., Tulunay, A. et al. Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet’s disease etiopathogenesis. Rheumatol Int 28, 401–406 (2008). https://doi.org/10.1007/s00296-007-0470-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00296-007-0470-y

Keywords

Search

Navigation