Abstract
Background
The pathogenesis of Henoch Schonlein Purpura is incompletely understood and the role of chemokines is unknown.
Objective
To investigate the levels of CC chemokines, eotaxin, TARC, and CXC chemokine IP-10 in Henoch Schonlein Purpura.
Methods
Three groups of children were enrolled in the study: Henoch Schonlein Purpura in active stage (n = 26), Henoch Schonlein Purpura in remission phase (n = 26) and healthy children (n = 18). Levels of eotaxin, TARC, and IP-10 were determined in plasma using ELISA.
Results
No significant difference was observed in the plasma level of eotaxin and TARC levels between the HSP and healthy children (>0.05). We could not find any significant difference between acute phase of the disease and convalescent phase in eotaxin and TARC levels (P > 0.05). We have suggested significant decreases in plasma IP-10 in the acute phase of the disease compared with the convalescent phase (P < 0.05). There was a significant difference in IP-10 levels between active stage and healthy controls, too (<0.05). We could not find any significant correlation between chemokine levels and system involvement (>0.05).
Conclusion
Our study shows that plasma level of eotaxin and TARC levels do not differ between the HSP and healthy children. But, decreasing the release of the Th1 chemokine IP-10 in HSP active stage may show that in HSP, there is no shift to Th1 lymphocytes in children with HSP. Further investigations are warranted to more fully explore and understand the production of and potential role of these chemokines in HSP.
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References
Cassidy JT, Petty RE (2001) Vasculitis, Henoch-Schonlein purpura. In: Cassidy JT, Petty E (eds) Pediatric rheumatology, 4th edn. Saunders, Philadelphia pp 569–579
Leung DY (1990) Immunologic aspects of Kawasaki syndrome. J Rheumatol Suppl 24:15–18
Cohen TJ, Kallenberg CG (1991) Vasculitis. In: The TH, De Kallenberg CGM, Leij L (eds) Klinische Immunologie. Houten/Antwerpen, Bohn Stafleu Van Loghum, pp 216–233
Ekenstam EA, Callen JP (1984) Cutaneous leukocytoclastic vasculitis. Clinical and laboratory features of 82 patients seen in private practice. Arch Dermatol 120:484–489
McKee PH, Calonje E, Granter SR (2005) Vascular diseases, Leukocytoclastic vasculitis. In: McKee PH, Calonje E, Granter SR (eds) Pathology of the skin, 3rd edn. Mosby, Philadelphia, pp 709–775
Schall TJ (1994) The chemokines. In: The chemokine handbook, 2nd edn. Academic, New York, pp 419–460
Ueno Y, Takano N, Kanegane H, Yokoi T, Yachie A, Miyawaki T, Taniguchi N (1989) The acute phase nature of interleukin-6: studies in Kawasaki disease and other febrile illnesses. Clin Exp Immunol 76:337–342
Kim HS, Kim WD, Lee YH (2003) Production and expression of Groand RANTES by peripheral blood mono nuclear cells isolated from patients with kawasaki disease and measles. J Korean Med Sci 18:381–386
Richmond A, Shattuck RL (1966) Melanoma growth stimulatory activity: physiology, biology, structure/function and role in disease. In: Horyk R (ed) Chemoattractant Ligands and their receptors, CRS Press, Boca Raton, pp 87–124
Mahanty S, Bausch DG, Thomas RL, Goba A, Bah A, Peters CJ, Rollin PE (2001) Low levels of interleukin-8 and interferon-inducible protein-10 in serum are associated with infections in acute Lassa fever. J Infect Dis 183:1713–1721
Frangogiannis NG, Mendoza LH, Smith CW, Michael LH, Entman ML (2000) Induction of the synthesis of the C-X-C chemokine interferon γ inducible protein-10 in experimental canine endotoxemia. Cell Tissue Res 302:365–376
Miotto D, Christodopoulos P, Olivenstein R (2001) Expression of IFN-gamma-inducible protein; monocyte chemotactic proteins 1, 3, and 4; and eotaxin in Th1 and Th2-mediated lung diseases. J Allergy Clin Immunol 107:664–670
Borish LC, Steinke JW (2003) Cytokines and chemokines. J Allergy Clin Immunol 111:S460–S475
Luster AD (1998) Chemokines—chemotactic cytokines that mediate inflammation. N Engl J Med 338:436–445
Davin JC, Berge IJT, Weenig JJ (2001) What is the difference between IgA nephropathy and Henoch-Schonlein purpura nephritis? Kidney Int 59:823–834
Chung HS, Kim HY, Kim HS, Lee HJ, Yuh JH, Lee ES, Choi KH, Lee YH (2004) Production of chemokines in Kawasaki disease, Henoch-Schonlein purpura and acute febrile illness. J Korean Med Sci 19:800–804
Yang YH, Lai HJ, Huang CM, Wang LC, Lin YT, Chiang BL (2004) Sera from children with active Henoch Schonlein purpura can enhance the production of interleukin 8 by human umblical venous endothelial cells. Ann Rheum Dis 63:1511–1513
Amoli MM, Thomson W, Hajeer AH, Calvino MC, Garcia-Porrua C, Ollier WE, Gonzalez-Gay MA (2002) Interleukin 8 gene polymorphism is associated with increased risk of nephritis in cutaneous vasculitis. J Rheumatol 29:2367–2370
Mills JA, Michel BA, Block DA, Calabrese LH, Hunder GG, Arend WP (1990) The American College of Rheumatology 1990 criteria for the classification of Henoch-Schonlein purpura. Arthritis Rheum 33:1114–1121
Besbas N, Saatci U, Ruacan S (1997) The role of cytokines in Henoch Schonlein purpura. Scand J Rheumatol 26:456–460
Bradley JR, Lockwood CM, Thiru S (1994) Endotheial cell activation in patients with systemic vasculitis. Q J Med 87:741–745
Butcher EC, Picker LJ (1996) Lymphocyte homing and homeostasis. Science 272:60
Baggiolini M (1998) Chemokines and leukocyte traffic. Nature 392:565
Quin S, Rottman JB, Myers N, Kassam N, Weinblatt M, Loetscher M, Koch AE, Moser B, Mackay CR (1998) The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. J Clin Invest 101:746
Bandeira-Melo C, Herbst A, Weller PF (2001) Eotaxins contributing to the diversity of eosinophil recruitment and activation. Am J Respir Cell Mol Biol 24:653–657
Teruya-Feldstein J, Jaffe ES, Burd PR, Kingma DW, Setsuda JE, Tosato G (1999) Differential chemokine expression in tissues involved by Hodgkin’s disease: direct correlation of eotaxin expression and tissue eosinophilia. Blood 93:2463–2470
Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, Celestin J, Leder P, Luster AD (1996) Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nature Med 97:604–612
Ying S, Robinson DS, Meng Q, Barata LT, McEuen AR, Buckley MG, Walls AF, Askenase PW, Kay AB (1999) CC chemokines in allergen induced late phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia and relationship to basophils and other CC chemokines (monocyte chemoattractant protein-3 and RANTES). J Immunol 163:3976–3984
Namgoong MK, Lim BK, Kim JS (1997) Eosinophilic cationic protein in Henoch-Schonlein purpura and IgA nephrophaty. Pediatr Nephrol 11:703–706
Kawasaki Y, Hosoya M, Suzuki H (2005) Possible pathogenic role of IL-5 and eosino cationic protein in Henoch-Schonlein purpura nephritis. Pediatr Int 47:512–517
Rankin SM, Conroy DM, Williams TJ (2000) Eotaxin and eosinophil recruitment: implications for human disease. Mol Med Today 6:20–27
Imai T, Baba M, Nishimura M, Kakizaki M, Takagi S, Yoshie O (1997) The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem 272:15036
Imai T, Takagi S, Nishimura M, Kakizaki M, Nishimura M, Wang J, Gray PW, Matsushima K, Yoshie O (1999) Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. Int Immunol 11:81
Bonecchi R, Bianchi G, Bordignon PP, D’Ambrosio D, Lang R, Borsatti A, Sozzani S, Allavena P, Gray A, Mantovani A (1998) Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s. J Exp Med 187:129
Sallusto F, Lenig D, Mackay R, Lanzavecchia A (1998) Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes. J Exp Med 187:875
Kakinuma T, Nakamura K, Wakugawa M, Mitsui H, Tada Y, Saeki H (2001) Thymus and activation-regulated chemokine in atopic dermatitis: serum thymus and activation-regulated chemokine level is closely related with disease activity. J Allergy Clin Immunol 107:535–541
Leung DYM (2000) Atopic dermatitis: new insights and opportunities for terapotic intervention. J Allergy Clin Immunol 105:860–876
Sekiya T, Yamada H, Yamaguchi M, Yamamoto K, Ishii A, Yoshie O, Sano Y, Morita A, Matsushima K, Hirai K (2002) Increased levels of Th2-type CC chemokine thymus and activation-regulated chemokine (TARC) in serum and induced sputum of asthmatics. Allergy 57:173–177
Medoff BD, Sauty A, Tager AM, Maclean JA, Smith RN, Mathew A, Dufour JH, Luster AD (2002) IFN-γ-inducble protein 10 (CXCL10) contributes to airway hyperreactivity and airway inflammation in a mouse model of asthma. J Immunol 168:5278–5286
Loetscher P, Pellegrino A, Gong JH, Mottioli I, Loetscher M, Bardi G, Baggiolini M, Clark-Lewis I (2001) The ligands of CXC chemokine receptor 3, I-TAC, Mig, and IP-10, are natural antagonists for CCR3. J Biological Chemist 276:2986–2991
Homey B, Zlotnik A (1999) Chemokines in allergy. Curr Opin Immunol 11:626
Sallusto F, Mackay CR, Lanzavecchia A (1997) Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science 277:2005
Siveke JT, Hamann A (1998) T helper 1 and T helper 2 cells respond differentially chemokines. J Immunol 160:550
Davin DJ, Pierard G, Dechenne C (1994) Possible pathogenic role of IgE in Henoch Schonlein purpura. Pediatr Nephrol 8:169–171
Davin DJ, Vandenbroek MC, Foidart JB (1985) Sequential measruments of the reticulo-endothelial system function in Henoch-Schonlein disease in childhood. Correlation with various immunological parameters. Acta Paediatr Scand 7:201–206
Urbanek R, Karitzky D, Kunzer W (1978) Serum IgE bei Hauterkrankungen im Kindersalter. Deutsche Med Wochenschr 103:503–508
Tsuji Y, Abe Y, Hisano M, Sakai T (2004) Urinary leukotriene E4 in Henoch-Schonlein purpura. Clin Exp Allergy 34:1259–1261
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Tahan, F., Dursun, I., Poyrazoglu, H. et al. The role of chemokines in Henoch Schonlein Purpura. Rheumatol Int 27, 955–960 (2007). https://doi.org/10.1007/s00296-007-0332-7
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DOI: https://doi.org/10.1007/s00296-007-0332-7