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Identification of the receptor for advanced glycation end products in synovial tissue of patients with rheumatoid arthritis

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Abstract

Objectives

Generation of advanced glycation end products (AGE) is an inevitable process in vivo and can be accelerated under pathologic conditions such as oxidative stress, e.g. in rheumatoid arthritis (RA). This process is mediated by the AGE-specific receptor (RAGE). In this study we analysed the presence of RAGE in RA and osteoarthritic (OA) synovial tissue using immunohistology.

Methods

Frozen synovial tissue samples from 11 RA patients and 12 OA patients were treated with goat anti-RAGE immunoglobulin G (IgG) and rabbit antigoat IgG. Immunostaining was visualised with streptavidin horse radish peroxidase (chromogen amino-ethyl-carbazole). Cell differentiation was performed with antibodies against CD68, CD45RO, and CD20.

Results

In 9/11 RA and 8/12 OA synovial specimens, RAGE was detected in synovial lining, sublining, and stroma. In RA, many T cells (CD45RO+) and some macrophages (CD68+) showed positive immunostaining for RAGE, whereas B cells were mostly negative. We found no difference in staining patterns between the RA and OA samples.

Conclusions

We detected RAGE in RA and OA synovial tissue. The presence of RAGE on macrophages, T cells, and some B cells suggests its role in the pathogenesis of inflammatory joint disease.

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Authors and Affiliations

  1. Department of Internal Medicine IV, Friedrich Schiller University, Jena, Germany

    Stefan Drinda, Sybille Franke, Michael Rüster, Günter Stein & Gert Hein

  2. Institute of Pathology, Friedrich Schiller University, Jena, Germany

    Peter Petrow

  3. Division of Orthopedic Rheumatology, University of Erlangen-Nuremnberg, Erlangen, Germany

    Oliver Pullig

  4. Klinik für Innere Medizin IV, Rheumatologie/Osteologie, Friedrich- Schiller- Universität, Erlanger Allee 101, 07740, Jena, Germany

    Stefan Drinda

Authors
  1. Stefan Drinda
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  2. Sybille Franke
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  3. Michael Rüster
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  4. Peter Petrow
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  5. Oliver Pullig
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  6. Günter Stein
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  7. Gert Hein
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Correspondence to Stefan Drinda.

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Drinda, S., Franke, S., Rüster, M. et al. Identification of the receptor for advanced glycation end products in synovial tissue of patients with rheumatoid arthritis. Rheumatol Int 25, 411–413 (2005). https://doi.org/10.1007/s00296-004-0456-y

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  • DOI: https://doi.org/10.1007/s00296-004-0456-y

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