Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p

Abstract.

Tup1p is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tup1p mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tup1p-interacting protein in this screen. Expression analysis of a strain deleted for CDC73 on a genome-wide scale revealed that Cdc73p is involved in the positive and negative regulation of transcription. Our results show that a subset of genes is regulated by both Cdc73p and Tup1p. Furthermore, our results indicate that Cdc73p plays a role in the repression of telomere-proximal genes, which are not repressed by Tup1p.