Skip to main content
SpringerLink
Log in

Cloning and heterologous expression of Solorina crocea pyrG

  • ORIGINAL PAPER
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

A pyrG gene, encoding orotidine 5′-monophosphate decarboxylase, was cloned from a phage library derived from the lichen Solorina crocea. Phylogenetic analysis and a survey of geographically well-separated specimens were used to verify that the gene represented the fungal component of the lichen. Both coding and upstream sequences of S. crocea pyrG exhibited features typical of fungal genes. A 132-bp intron interrupting the coding region between nucleotides 157 and 288 was confirmed by RT-PCR and sequencing. Transformation of Aspergillus nidulans with S. crocea pyrG, controlled by either its native promoter or the A. nidulans trpC promoter, resulted in uridine-independent strains that exhibited appreciable growth only at 24 °C. Southern analysis indicated multiple integrations of S. crocea pyrG. These results demonstrate that heterologous expression may be used to investigate genes from lichens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. TerraGen Discovery, Inc., 300-2386 East Mall – UBC, Vancouver British Columbia, V6T 1Z3, Canada, , , , , , CA

    Shannon J. Sinnemann, Daren W. Brown & Vivian P. W. Miao

  2. Institute for Experimental Pathology, University of Iceland, Keldur v/Vesturlandsveg, IS-112 Reykjavík, Iceland, , , , , , IS

    Ólafur S. Andrésson

Authors
  1. Shannon J. Sinnemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ólafur S. Andrésson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daren W. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vivian P. W. Miao
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 1 December 1999 / 10 February 2000

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sinnemann, S., Andrésson, Ó., Brown, D. et al. Cloning and heterologous expression of Solorina crocea pyrG. Curr Genet 37, 333–338 (2000). https://doi.org/10.1007/s002940050536

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002940050536

Search

Navigation