Abstract
The yeast non-Mendelian [PSI +] determinant is presumed to be the manifestation of the aggregated prion-like state of the Sup35 protein. Plasmid-mediated amplification of the SUP35 gene greatly increases the frequency of Sup35p transition to this prion-like state. Here we show that the 3′-deletions of plasmid SUP35, leading to the C-terminal truncation of Sup35p, further increase the frequency of [PSI +] induction despite a marked decrease in Sup35p expression levels. The data suggest that the presence of Sup35p N-terminal proteolytic fragments can cause [PSI +] appearance in wild-type yeast cells.
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Received: 2 February / 24 June 1998
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Kochneva-Pervukhova, N., Poznyakovski, A., Smirnov, V. et al. C-terminal truncation of the Sup35 protein increases the frequency of de novo generation of a prion-based [PSI+] determinant in Saccharomyces cerevisiae. Curr Genet 34, 146–151 (1998). https://doi.org/10.1007/s002940050379
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DOI: https://doi.org/10.1007/s002940050379