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Chaperoning RPA during DNA metabolism

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Abstract

Single-stranded DNA (ssDNA) is widely generated during DNA metabolisms including DNA replication, repair and recombination and is susceptible to digestion by nucleases and secondary structure formation. It is vital for DNA metabolism and genome stability that ssDNA is protected and stabilized, which are performed by the major ssDNA-binding protein, and replication protein A (RPA) in these processes. In addition, RPA-coated ssDNA also serves as a protein–protein-binding platform for coordinating multiple events during DNA metabolisms. However, little is known about whether and how the formation of RPA–ssDNA platform is regulated. Here we highlight our recent study of a novel RPA-binding protein, Regulator of Ty1 transposition 105 (Rtt105) in Saccharomyces cerevisiae, which regulates the RPA–ssDNA platform assembly at replication forks. We propose that Rtt105 functions as an “RPA chaperone” during DNA replication, likely also promoting the assembly of RPA–ssDNA platform in other processes in which RPA plays a critical role.

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Acknowledgements

This work was supported by grants from NSFC [31725015, 31830048 (QL) and 31671332 (JF)].

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  1. Shuqi Li and Ziqi Dong contributed equally.

Authors and Affiliations

  1. State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China

    Shuqi Li, Ziqi Dong, Shuangshuang Yang, Jianxun Feng & Qing Li

  2. Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China

    Shuqi Li & Qing Li

  3. Laboratory of Host-Pathogen Biology, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA

    Shuqi Li

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  1. Shuqi Li
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  2. Ziqi Dong
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Correspondence to Qing Li.

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Li, S., Dong, Z., Yang, S. et al. Chaperoning RPA during DNA metabolism. Curr Genet 65, 857–864 (2019). https://doi.org/10.1007/s00294-019-00945-3

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