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The influence of mitochondrial dynamics on mitochondrial genome stability

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Abstract

Mitochondria are dynamic organelles that fuse and divide. These changes alter the number and distribution of mitochondrial structures throughout the cell in response to developmental and metabolic cues. We have demonstrated that mitochondrial fission is essential to the maintenance of mitochondrial DNA (mtDNA) under changing metabolic conditions in wild-type Saccharomyces cerevisiae. While increased loss of mtDNA integrity has been demonstrated for dnm1-∆ fission mutants after growth in a non-fermentable carbon source, we demonstrate that growth of yeast in different carbon sources affects the frequency of mtDNA loss, even when the carbon sources are fermentable. In addition, we demonstrate that the impact of fission on mtDNA maintenance during growth in different carbon sources is neither mediated by retrograde signaling nor mitophagy. Instead, we demonstrate that mitochondrial distribution and mtDNA maintenance phenotypes conferred by loss of Dnm1p are suppressed by the loss of Sod2p, the mitochondrial matrix superoxide dismutase.

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Acknowledgements

E.A.S and R.A.S research was supported by National Science Foundation Grants, MCB0841857 and MCB1243428. E.A.S. and C.T.P. received additional support from University of Rochester Pump Primer Grant, OP212588.

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Authors and Affiliations

  1. Department of Biology, University of Rochester, Rochester, NY, USA

    Christopher T. Prevost & Elaine A. Sia

  2. Department of Biology, The College at Brockport, State University of New York, Brockport, NY, USA

    Christopher T. Prevost, Nicole Peris, Christina Seger, Deanna R. Pedeville, Kathryn Wershing & Rey A. L. Sia

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  1. Christopher T. Prevost
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  2. Nicole Peris
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  3. Christina Seger
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  4. Deanna R. Pedeville
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  5. Kathryn Wershing
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  6. Elaine A. Sia
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  7. Rey A. L. Sia
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Correspondence to Elaine A. Sia or Rey A. L. Sia.

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Communicated by M. Kupiec.

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Prevost, C.T., Peris, N., Seger, C. et al. The influence of mitochondrial dynamics on mitochondrial genome stability. Curr Genet 64, 199–214 (2018). https://doi.org/10.1007/s00294-017-0717-4

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  • DOI: https://doi.org/10.1007/s00294-017-0717-4

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