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Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria

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Abstract

Type-II NAD(P)H dehydrogenases form a multigene family that comprise six members in the green microalga Chlamydomonas. To date, only one enzyme (Nda2) located in the chloroplast has been characterized in this alga and demonstrated to participate in the reduction of the plastoquinone pool. We present here the functional characterization of Nda1. The enzyme is located on the inner face of the inner mitochondrial membrane. Its downregulation leads to a slight decrease of NADH:ferricyanide activity and of dark whole cell respiration. To determine whether the reduction of Nda1 combined with the lack of complex I would affect mitochondrial processes, double mutants affected in both Nda1 and complex I were isolated. Respiration and growth rates in heterotrophic conditions were significantly altered in the double mutants investigated, suggesting that Nda1 plays a role in the oxidation of matrix NADH in the absence of complex I.

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Acknowledgments

We thank Michele Radoux and Valerie Roels for expert technical assistance. This work was supported by Action de Recherche Concertee ARC07/12-04, an FP7-funded project (Sunbiopath, GA245070), Fonds National de Recherche Scientifique (FRFC 2.4567.11 and 2.4597.11) and les ‘Fonds spéciaux de l’Université de Liège’. Pierre Cardol is a research associate from FNRS.

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  1. Laboratory of Genetics of Microorganisms, Institute of Botany, University of Liege, B22, Boulevard du Rectorat, 27, 4000, Liege, Belgium

    Renaud Lecler, Hélène Vigeolas, Barbara Emonds-Alt, Pierre Cardol & Claire Remacle

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  1. Renaud Lecler
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  2. Hélène Vigeolas
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  3. Barbara Emonds-Alt
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  5. Claire Remacle
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Correspondence to Claire Remacle.

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Communicated by A. Grossman.

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Lecler, R., Vigeolas, H., Emonds-Alt, B. et al. Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria. Curr Genet 58, 205–216 (2012). https://doi.org/10.1007/s00294-012-0378-2

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