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Extracellular proteome of Aspergillus terreus grown on different carbon sources

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Abstract

Extracellular proteins of filamentous fungi are important for biomedical and biotechnological applications. Aspergillus terreus not only comprises an important class of organisms that have significant commercial relevance to the biotechnology industry, but also is an emerging fungal pathogen. However, no information is available on the extracellular proteome of A. terreus. Thus, we analyzed the extracellular proteomes of A. terreus under different culture conditions using sucrose, glucose, or starch as a main carbon source. A total of 82 protein spots including 39 unique proteins was successfully identified by 2-DE and nano-LC-MS/MS. Of these, 12 proteins were detected in the presence of at least two different carbon sources, whereas 16 proteins were unique to sucrose-, 3 to glucose-, and 8 to starch-grown A. terreus. Most of the proteins with known functions are hydrolytic enzymes, such as hydrolases, glycosylases and proteases, some of which include potential allergens. Both oryzin and a predicted protein (ATEG_07481) were the most abundant in all three media. Particularly, oryzin was highly secreted in high concentration sucrose medium. These proteomic data will be useful for studying protein secretion in further detail, and finding fusion partners for the extracellular production of homologous or heterologous proteins in A. terreus.

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Acknowledgments

This work was supported by the Korean Systems Biology Research Grant (20090065571) of Ministry of Education, Science and Technology (MEST) to S.Y. Lee through the National Research Foundation (NRF) and the Converging Research Center Program from the NRF (2009-0093652) to M.-J. Han. Further supports by the World Class University Program (R32-2008-000-10142-0) of the MEST through the NRF, LG Chem Chair Professorship, IBM SUR program, and Microsoft are appreciated.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology and Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Mee-Jung Han, Nag-Jong Kim, Sang Yup Lee & Ho Nam Chang

  2. Department of Biomolecular and Chemical Engineering, Dongyang University, 1 Gyochon-dong, Punggi-eup, Yeongju, Gyeongbuk, 750-711, Republic of Korea

    Mee-Jung Han

  3. Department of Bio and Brain Engineering, KAIST, Daejeon, Republic of Korea

    Sang Yup Lee

  4. Department of Biological Sciences, and Bioinformatics Research Center, KAIST, Daejeon, Republic of Korea

    Sang Yup Lee

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  1. Mee-Jung Han
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  2. Nag-Jong Kim
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  3. Sang Yup Lee
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Correspondence to Mee-Jung Han or Sang Yup Lee.

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Communicated by U. Kueck.

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Han, MJ., Kim, NJ., Lee, S.Y. et al. Extracellular proteome of Aspergillus terreus grown on different carbon sources. Curr Genet 56, 369–382 (2010). https://doi.org/10.1007/s00294-010-0308-0

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