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Development of a molecular genetic linkage map for Colletotrichum lindemuthianum and segregation analysis of two avirulence genes

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Abstract

A framework genetic map was developed for the fungal pathogen Colletotrichum lindemuthianum, the causal agent of anthracnose of common bean (Phaseolus vulgaris L.). This is the first genetic map for any species within the family Melanconiaceae and the genus Colletotrichum and provides the first estimate of genome length for C. lindemuthianum. The map was generated using 106 haploid F1 progeny derived from crossing two Mexican C. lindemuthianum isolates differing in two avirulence genes (AvrclMex and AvrclTO). The map comprises 165 AFLP markers covering 1,897 cM with an average spacing of 11.49 cM. The markers are distributed over 19 major linkage groups containing between 5 and 25 markers each and the genome length was estimated to be approximately 3,241 cM. The avirulence genes AvrclMex and AvrclTO segregate in a 1:1 ratio supporting the gene for gene hypothesis for the incompatible reaction between C. lindemuthianum and P. vulgaris, but could not be incorporated into the genetic map. This initial outline map forms the basis for the development of a more detailed C. lindemuthianum linkage map, which would include other types of molecular markers and allow the location of genes previously isolated and characterized in this species.

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Acknowledgments

We are grateful to Emigdia Alfaro for excellent technical assistance in the AFLP analysis, Octavio Martínez de la Vega for helpful discussions on the statistical and mapping analysis and to Luis Herrera-Estrella for critical reading of the manuscript. This research was supported by grants from CONACyT/SAGAR no. K159-A9702 and SEP-CONACyT grant nos. 28275 and 40369-Q. FLM is also indebted to CONACyT, Mexico for doctoral fellowship no. 120910.

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Authors and Affiliations

  1. Department of Genetic Engineering, CINVESTAV, Unidad Irapuato, Apdo. Postal 629, Irapuato, Guanajuato, México

    Francisco Luna-Martínez, Mayra Victoria-Campos & June Simpson

  2. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Apdo. Postal 112, Campo Experimental Bajío, Celaya, Guanajuato, México

    Raúl Rodríguez-Guerra

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  1. Francisco Luna-Martínez
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  2. Raúl Rodríguez-Guerra
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  3. Mayra Victoria-Campos
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  4. June Simpson
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Correspondence to June Simpson.

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Communicated by U. Kück.

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Luna-Martínez, F., Rodríguez-Guerra, R., Victoria-Campos, M. et al. Development of a molecular genetic linkage map for Colletotrichum lindemuthianum and segregation analysis of two avirulence genes. Curr Genet 51, 109–121 (2007). https://doi.org/10.1007/s00294-006-0111-0

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