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Mosaic (MSC) cucumbers regenerated from independent cell cultures possess different mitochondrial rearrangements

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Abstract

Passage of the highly inbred cucumber (Cucumis sativus L.) line B through cell culture produces progenies with paternally transmitted, mosaic (MSC) phenotypes. Because the mitochondrial genome of cucumber shows paternal transmission, we evaluated for structural polymorphisms by hybridizing cosmids spanning the entire mitochondrial genome of Arabidopsis thaliana L. to DNA-gel blots of four independently generated MSC and four wild-type cucumbers. Polymorphisms were identified by cosmids carrying rrn18, nad5-exon2, rpl5, and the previously described JLV5 deletion. Polymorphisms revealed by rrn18 and nad5-exon2 were due to one rearrangement bringing together these two coding regions. The polymorphism revealed by rpl5 was unique to MSC16 and was due to rearrangement(s) placing the rpl5 region next to the forward junction of the JLV5 deletion. The rearrangement near rpl5 existed as a sublimon in wild-type inbred B, but was not detected in the cultivar Calypso. Although RNA-gel blots revealed reduced transcription of rpl5 in MSC16 relative to wild-type cucumber, Western analyses revealed no differences for the RPL5 protein and the genetic basis of the MSC16 phenotype remains enigmatic. We evaluated 17 MSC and wild-type lines regenerated from independent cell-culture experiments for these structural polymorphisms and identified eight different patterns, indicating that the passage of cucumber through cell culture may be a unique mechanism to induce or select for novel rearrangements affecting mitochondrial gene expression.

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Acknowledgements

We thank Drs. Joseph Walker (University of Wisconsin, USA) for help with protein analyses, Tom Elthon (University of Nebraska, USA) for providing the anti-PORIN mouse monoclonal antibody, Axel Brennicke (Universität Ulm, Germany) for the Arabidopsis mitochondrial cosmids, and Hanna Janska (Uniwersytet Wrocławski, Poland) for critical reading of this manuscript. G.B. was partially supported by the NATO Advanced Fellowships Programme for post-doctoral research at the University of Wisconsin. Product names are necessary to report factually on available data. However, the United States Department of Agriculture (USDA) neither guarantees nor warrants the standard of the product; and the use of a name by the USDA implies no approval of the product to the exclusion of others that may also be suitable.

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Authors and Affiliations

  1. Vegetable Crops Unit, Department of Horticulture, Agricultural Research Service, U.S. Department of Agriculture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA

    Grzegorz Bartoszewski & Michael J. Havey

  2. Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape Architecture, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787, Warsaw, Poland

    Grzegorz Bartoszewski & Stefan Malepszy

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  1. Grzegorz Bartoszewski
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  2. Stefan Malepszy
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  3. Michael J. Havey
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Correspondence to Michael J. Havey.

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Communicated by A. Brennicke

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Bartoszewski, G., Malepszy, S. & Havey, M.J. Mosaic (MSC) cucumbers regenerated from independent cell cultures possess different mitochondrial rearrangements. Curr Genet 45, 45–53 (2004). https://doi.org/10.1007/s00294-003-0456-6

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