Abstract
Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites (CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.
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Acknowledgements
This work was supported in part by funds from National Institutes of Health grant AI40320 to J.S.K. and USUHS grants R073AM and R073IF to S.G.L. These data were submitted to the State University of New York-Albany in partial fulfillment for a PhD degree in Biomedical Sciences (C.E.R.) and have been presented in part at national and international meetings. At the Wadsworth Center, we thank the Electron Microscopy, Photo/Illustration, Molecular Genetics, and Peptide Synthesis Core Facilities, Fogarty Fellow Dr. J.R. Slapeta for assisting with GFP/MitoTracker red double-labeling yeast mitochondria, and members of the dissertation committee for many helpful comments. At Rockefeller University, we thank Dr. L.B. Sanchez for assistance with the phylogenetic analyses and Dr. M. Muller for critically reviewing the manuscript.
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Riordan, C.E., Ault, J.G., Langreth, S.G. et al. Cryptosporidium parvum Cpn60 targets a relict organelle. Curr Genet 44, 138–147 (2003). https://doi.org/10.1007/s00294-003-0432-1
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DOI: https://doi.org/10.1007/s00294-003-0432-1