Skip to main content
SpringerLink
Log in

Genetic conservation versus variability in mitochondria: the architecture of the mitochondrial genome in the petite-negative yeast Schizosaccharomyces pombe

  • Review Article
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

The great amount of molecular information and the many molecular genetic techniques available make Schizosaccharomyces pombe an ideal model eukaryote, complementary to the budding yeast Saccharomyces cerevisiae. In particular, mechanisms involved in mitochiondrial (mt) biogenesis in fission yeast are more similar to higher eukaryotes than to budding yeast. In this review, recent findings on mt morphogenesis, DNA replication and gene expression in this model organism are summarised. A second aspect is the organisation of the mt genome in fission yeast. On the one hand, fission yeast has a strong tendency to maintain mtDNA intact; and, on the other hand, the mt genomes of naturally occurring strains show a great variability. Therefore, the molecular mechanisms behind the susceptibility to mutations in the mtDNA and the mechanisms that promote sequence variations during the evolution of the genome in fission yeast mitochondria are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

References

  • Adams AE, Pringle JR (1984) Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. J Cell Biol 98:934–945

    CAS  PubMed  Google Scholar 

  • Ahne A, Mueller-Derlich J, Merlos-Lange AM, Kanbay F, Wolf K, Lang BF (1988) Two distinct mechanisms for deletion in mitochondrial DNA of Schizosaccharomyces pombe mutator strains. Slipped mispairing mediated by direct repeats and erroneous intron splicing. J Mol Biol 202:725–734

    CAS  PubMed  Google Scholar 

  • Belfort M, Perlman PS (1995) Mechanisms of intron mobility. J Biol Chem 270:30237–30240

    Article  CAS  PubMed  Google Scholar 

  • Bendich AJ (1993) Reaching for the ring: the study of mitochondrial genome structure. Curr Genet 24:279–290

    Google Scholar 

  • Bendich AJ (1996) Structural analysis of mitochondrial DNA molecules from fungi and plants using moving pictures and pulsed-field electrophoresis. J Mol Biol 255:564–588

    Article  CAS  PubMed  Google Scholar 

  • Berger KH, Yaffe MP (1996) Mitochondrial distribution and inheritance. Experientia 52:1111–1116

    CAS  PubMed  Google Scholar 

  • Berger KH, Sogo LF, Yaffe MP (1997) Mdm12p is a component required for mitochondrial inheritance that is conserved between budding and fission yeast. J Cell Biol 136:545–553

    Article  CAS  PubMed  Google Scholar 

  • Bostock CH (1969) Mitochondrial DNA in the fission yeast Schizosaccharomyces pombe. Biochim Biophys Acta 195:579–581

    Article  CAS  PubMed  Google Scholar 

  • Boulet A, Levra-Juillet E, Perea J, Faye G (1990) The absence of introns in yeast mitochondria does not abolish mitochondrial recombination. Curr Genet 18:295–299

    Google Scholar 

  • Bulder CJ (1964a) Induction of petite mutation and inhibition of synthesis of respiratory enzymes in various yeasts. Antonie van Leeuwenhoek 30:1–9

    CAS  Google Scholar 

  • Bulder CJ (1964b) Lethality of the petite mutation in petite negative yeasts. Antonie van Leeuwenhoek 30:442–454

    CAS  Google Scholar 

  • Bullerwell CE, Burger G, Lang BF (2000) A novel motif for identifying Rps3 homologs in fungal mitochondrial genomes. Trends Biochem Sci 25:363–365

    Article  CAS  PubMed  Google Scholar 

  • Bullerwell CE, Leigh J, Forget L, Lang BF (2003) A comparison of three fission yeast mitochondrial genomes. Nucleic Acids Res 31:759–768

    Article  CAS  PubMed  Google Scholar 

  • Chen XJ, Clark-Walker GD (1993) Mutations in MGI genes convert Kluyveromyces lactis into a petite-positive yeast. Genetics 133:517–525

    CAS  PubMed  Google Scholar 

  • Chen XJ, Clark-Walker GD (1995) Specific mutations in alpha- and gamma-subunits of F1-ATPase affect mitochondrial genome integrity in the petite-negative yeast Kluyveromyces lactis. EMBO J 14:3277–3286

    CAS  PubMed  Google Scholar 

  • Chen XJ, Clark-Walker GD (1999a) Alpha and beta subunits of F1-ATPase are required for survival of petite mutants in Saccharomyces cerevisiae. Mol Gen Genet 262:898–908

    Article  CAS  PubMed  Google Scholar 

  • Chen XJ, Clark-Walker GD (1999b) The petite mutation in yeasts: 50 years on. Int Rev Cytol 194:195–236

    Google Scholar 

  • Chen XJ, Hansbro PM, Clark-Walker GD (1998) Suppression of rhoo lethality by mitochondrial ATP synthase F1 mutations in Kluyveromyces lactis occurs in the absence of F0. Mol Gen Genet 259:457–467

    Article  CAS  PubMed  Google Scholar 

  • Clark-Walker GD, Hansbro PM, Gibson F, Chen XJ (2000) Mutant residues suppressing rho(0)-lethality in Kluyveromyces lactis occur at contact sites between subunits of F(1)-ATPase. Biochim Biophys Acta 1478:125–137

    Article  CAS  PubMed  Google Scholar 

  • Davidson MT, Garland PB (1977) Structure of mitochondria and vacuoles of Candida utilis and Schizosaccharomyces pombe studied by electron microscopy of serial thin sections and model building. J Gen Microbiol 98:147–152

    PubMed  Google Scholar 

  • De Deken RH (1961) The dissociation of phenotypic and inheritable effects of euflavin in yeast. Exp Cell Res 24:145–148

    Google Scholar 

  • De Deken RH (1966) The Crabtree effect: a regulatory system in yeast. J Gen Microbiol 44:149–156

    PubMed  Google Scholar 

  • Del Giudice L, Wolf K (1980) Evidence for a joint control of nuclear and mitochondrial DNA synthesis in the petite negative yeast Schizosaccharomyces pombe. In: Schwemmler W, Schenk HEA (eds) Endosymbiosis and cell research. Elsevier, New York, pp 775–79

  • Del Giudice L, Wolf K, Buono C, Manna F (1981a) Nucleo-cytoplasm interactions in the petite negative yeast Schizosaccaromyces pombe: inhibition of nuclear and mitochondrial DNA synthesis in the absence of cytoplasmatic protein synthesis. Mol Gen Genet 181:306–308

    PubMed  Google Scholar 

  • Del Giudice L, Wolf K, Manna F, Pagliuca N (1981b) Synthesis of mitochondrial DNA during the cell cycle of the petite negative yeast Schizosaccharoymces pombe. Mol Gen Genet 182:252–254

    PubMed  Google Scholar 

  • Desjardins P, Frost E, Morais R (1985) Ethidium bromide-induced loss of mitochondrial DNA from primary chicken embryo fibroblasts. Mol Cell Biol 5:1163–1169

    CAS  PubMed  Google Scholar 

  • Desjardins P, Muys JM de, Morais R (1986) An established avian fibroblast cell line without mitochondrial DNA. Somat Cell Mol Genet 12:133–139

    CAS  PubMed  Google Scholar 

  • Doe CL, Osman F, Dixon J, Whitby MC (2000) The Holliday junction resolvase SpCCE1 prevents mitochondrial DNA aggregation in Schizosaccharomyces pombe. Mol Gen Genet 263:889–897

    Article  CAS  PubMed  Google Scholar 

  • Dujon B (1989) Group I introns as mobile genetic elements: facts and mechanistic speculations—a review. Gene 82:91–114

    CAS  PubMed  Google Scholar 

  • Eskes R, Yang J, Lambowitz AM, Perlman PS (1997) Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing. Cell 88:865–874

    CAS  PubMed  Google Scholar 

  • Eskes R, Liu L, Ma H, Chao MY, Dickson L, Lambowitz AM, Perlman PS (2000) Multiple homing pathways used by yeast mitochondrial group II introns. Mol Cell Biol 20:8432–8446

    Article  CAS  PubMed  Google Scholar 

  • Ferat JL, Michel F (1993) Group II self-splicing introns in bacteria. Nature 364:358–361

    CAS  PubMed  Google Scholar 

  • Forsburg SL (1999) The best yeast? Trends Genet 15:340–344

    Google Scholar 

  • Forsburg SL, Nurse P (1991) Cell cycle regulation in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Annu Rev Cell Biol 7:227–256

    Article  CAS  PubMed  Google Scholar 

  • Fox TD, Weiss-Brummer B (1980) Leaky +1 and −1 frameshift mutations at the same site in a yeast mitochondrial gene. Nature 288:60–63

    CAS  PubMed  Google Scholar 

  • Gargouri A, Lazowska J, Slonimski PP (1983) DNA-splicing of introns in a gene: a general way of reverting intron mutations. In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria. de Gruyter, Berlin, pp 259–268

  • Giraud MF, Velours J (1997) The absence of the mitochondrial ATP synthase delta subunit promotes a slow growth phenotype of rho− yeast cells by a lack of assembly of the catalytic sector F1. Eur J Biochem 245:813–818

    CAS  PubMed  Google Scholar 

  • Goffeau A, Briquet M, Colson AM, Delhez J, Foury F, Labaille F, Landry Y, Mohar O, Mrena E (1973) Stable pleiotrophic respiratory-deficient mutants of a "petite-negative" yeast: Schizosaccharomyces pombe as new tools to study the nuclear control of the assembly of the inner mitochondrial membrane. In: Tzagoloff A (ed) Membrane biogenesis. Plenum, New York, pp 63–97

  • Gray MW, Sankoff D, Cedergren RJ (1984) On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in the small subunit ribosomal RNA. Nucleic Acids Res 12:5837–5852

    CAS  PubMed  Google Scholar 

  • Haffter P, Fox TD (1992) Nuclear mutations in the petite-negative yeast Schizosaccharomyces pombe allow growth of cells lacking mitochondrial DNA. Genetics 131:255–260

    CAS  PubMed  Google Scholar 

  • Han ZY, Stachow C (1994) Analysis of Schizosaccharomyces pombe mitochondrial DNA replication by two dimensional gel electrophoresis. Chromosoma 103:162–170

    Article  CAS  PubMed  Google Scholar 

  • Hermann GJ, Shaw JM (1998) Mitochondrial dynamics in yeast. Annu Rev Cell Dev Biol 14:265–303

    Article  CAS  PubMed  Google Scholar 

  • Heslot H, Goffeau A, Louis C (1970) Respiratory metabolism of a "petite negative" yeast Schizosaccharomyces pombe 972h−. J Bacteriol 104:473–481

    CAS  PubMed  Google Scholar 

  • Hikkel I, Gbelska Y, Aart QJ van der, Lubecu G, Subik J (1997) Cloning and characterization of KlCOX18, a gene required for activity of cytochrome oxidase in Kluyveromyces lactis. Curr Genet 32:267–272

    Article  CAS  PubMed  Google Scholar 

  • Ho Y, Kim SJ, Waring RB (1997) A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease. Proc Natl Acad Sci USA 94:8994–8999

    Article  CAS  PubMed  Google Scholar 

  • Hu JP, Vanderstraeten S, Foury F (1995) Isolation and characterization of ten mutator alleles of the mitochondrial DNA polymerase-encoding MIP1 gene from Saccharomyces cerevisiae. Gene 160:105–110

    Article  CAS  PubMed  Google Scholar 

  • Ikeda S and Kawasaki N (2001) Isolation and characterization of the Schizosaccharomyces pombe cDNA encoding the mitochondrial endonuclease(1). Biochim Biophys Acta 1519:111–116

    Article  CAS  PubMed  Google Scholar 

  • Ikeda S, Maeda N, Ohshima T, Takata N (1996) Identification and characterization of a mitochondrial endonuclease from yeast, Schizosaccharomyces pombe. Biochem Mol Biol Int 40:1017–1024

    CAS  PubMed  Google Scholar 

  • Jacobs MA, Payne SR, Bendich AJ (1996) Moving pictures and pulsed-field gel electrophoresis show only linear mitochondrial DNA molecules from yeasts with linear-mapping and circular-mapping mitochondrial genomes. Curr Genet 30:3–11

    Article  CAS  PubMed  Google Scholar 

  • Kanbe T, Kobayashi I, Tanaka K (1989) Dynamics of cytoplasmic organelles in the cell cycle of the fission yeast Schizosaccharomyces pombe: three-dimensional reconstruction from serial sections. J Cell Sci 94:647–656

    PubMed  Google Scholar 

  • Karlok MA, Jang SH, Jaehning JA (2002) Mutations in the yeast mitochondrial RNA polymerase specificity factor, Mtf1, verify an essential role in promoter utilization. J Biol Chem 277:28143–28149

    Article  CAS  PubMed  Google Scholar 

  • Kilmartin JV, Adams AE (1984) Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. J Cell Biol 98:922–933

    Google Scholar 

  • King MP, Attardi G (1989) Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science 1989:500–503

    Google Scholar 

  • Kleff S, Kemper B, Sternglanz R (1992) Identification and characterization of yeast mutants and the gene for a cruciform cutting endonuclease. EMBO J 11:699–704

    CAS  PubMed  Google Scholar 

  • Kominsky DJ, Thorsness PE (2000) Expression of the Saccharomyces cerevisiae gene YME1 in the petite-negative yeast Schizosaccharomyces pombe converts it to petite-positive. Genetics 154:147–154

    CAS  PubMed  Google Scholar 

  • Kominsky DJ, Brownson MP, Updike DL, Thorsness PE (2002) Genetic and biochemical basis for viability of yeast lacking mitochondrial genomes. Genetics 162:1595–1604

    CAS  PubMed  Google Scholar 

  • Kotylak Z, Lazowska J, Hawthorne DC, Slonimski PP (1985) Intron encoded proteins of mitochondria: key elements of expression and genomic evolution. In: Quagliariello E, Slater EC, Palmeri F, Saccone C, Kroon AM (eds) Achievements and perspectives of mitochondrial research. Elsevier, New York, pp 1–21

  • Kovac L, Groot GS, Racker E (1972) Translocation of protons and potassium ions across the mitochondrial membrane of respiring and respiration-deficient yeasts. Biochim Biophys Acta 256:55–65

    Article  CAS  PubMed  Google Scholar 

  • Kovacova V, Irmlerova J, Kovac L (1968) Oxidative phosphorylatiion in yeast. IV. Combination of a nuclear mutation affecting oxidative phosphorylation with cytoplasmic mutation to respiratory deficiency. Biochim Biophys Acta 162:157–163

    CAS  PubMed  Google Scholar 

  • Kück U, Osiewacz HD, Schmidt U, Kappelhoff B, Schulte E, Stahl U, Esser K (1985) The onset of senescence is affected by DNA rearrangements of a discontinuous mitochondrial gene in Podospora anserina. Curr Genet 9:373–382

    PubMed  Google Scholar 

  • Labaille F, Colson AM, Petit L, Goffeau A (1977) Properties of a mitochondrial suppressor mutation restoring oxidative phosphorylation in a nuclear mutant of the yeast Schizosaccharomyces pombe. J Biol Chem 252:5716–5723

    CAS  PubMed  Google Scholar 

  • Lambowitz AM, Belfort M (1993) Introns as mobile genetic elements. Annu Rev Biochem Sci 62:587–622

    Article  CAS  Google Scholar 

  • Lang BF (1984) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: highly homologous introns are inserted at the same position of the otherwise less conserved cox1 genes in Schizosaccharomyces pombe and Aspergillus nidulans. EMBO J 3:2129–2136

    CAS  PubMed  Google Scholar 

  • Lang BF, Ahne F, Bonen L (1985) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe. The cytochrome b gene has an intron closely related to the first two introns in the Saccharomyces cerevisiae cox1 gene. J Mol Biol 184:353–366

    CAS  PubMed  Google Scholar 

  • Lang BF, Cedergren R, Gray MW (1987) The mitochondrial genome of the fission yeast, Schizosaccharomyces pombe. Sequence of the large-subunit ribosomal RNA gene, comparison of potential secondary structure in fungal mitochondrial large-subunit rRNAs and evolutionary considerations. Eur J Biochem 169:527–537

    CAS  PubMed  Google Scholar 

  • Lockshon D, Zweifel SG, Freeman-Cook LL, Lorimer HE, Brewer BJ, Fangman WL (1995) A role for recombination junctions in the segregation of mitochondrial DNA in yeast. Cell 81:947–955

    CAS  PubMed  Google Scholar 

  • Löffler M, Jockel J, Schuster G, Becker C (1997) Dihydroorotat-ubiquinone oxidoreductase links mitochondria in the biosynthesis of pyrimidine nucleotides. Mol Cell Biochem 174:125–129

    Article  PubMed  Google Scholar 

  • Manna F, Massardo DR, Del Giudice L, Buonocore A, Nappo AG, Alifano P, Schäfer B, Wolf K (1991) The mitochondrial genome of Schizosaccharomyces pombe: stimulation of intra-chromosomal recombination in E. coli by the gene product of the first cox1 intron. Curr Genet 19:295–299

    CAS  PubMed  Google Scholar 

  • Marchini A, Accardi R, Malanchi I, Schyr E, Oxelmark E, De Pal V, Jauniaux JC, Maundrell K, Tommasino M (2002) Schizosaccharomyces pombe Pmf1p is structurally and functionally related to Mmf1p of Saccharomyces cerevisiae. Yeast 19:703–711

    Article  CAS  PubMed  Google Scholar 

  • Michel F, Ferat JL (1995) Structure and activities of group II introns. Annu Rev Biochem 64:435–461

    Article  CAS  PubMed  Google Scholar 

  • Michel F, Umesono K, Ozeki H (1989) Comparative and functional anatomy of group II catalytic introns—a review. Gene 82:5–30

    CAS  PubMed  Google Scholar 

  • Monteilhet C, Dziadkowiec D, Szczepanek T, Lazowska J (2000) Purification and characterization of the DNA cleavage and recognition site of I-ScaI mitochondrial group I intron encoded endonuclease produced in Escherichia coli. Nucleic Acids Res 28:1245–1251

    Article  CAS  PubMed  Google Scholar 

  • Morais R, Desjardins P, Turmel C, Zinkewich-Peotti K (1988) Development and characterization of continuous avian cell lines depleted of mitochondrial DNA. In Vitro Cell Dev Biol Anim 24:649–658

    CAS  Google Scholar 

  • Mulder W, Scholten IH, Roon H van, Grivell LA (1994) Isolation and characterisation of the linked genes APA2 and QCR7, coding for Ap4A phosphorylase II and the 14 kDa subunit VII of the mitochondrial bc1-complex in the yeast Kluyveromyces lactis. Biochim Biophys Acta 1219:719–723

    Article  CAS  PubMed  Google Scholar 

  • Nagy M, Lacroute F, Thomas D (1992) Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts. Proc Natl Acad Sci USA 89:8966–8970

    CAS  PubMed  Google Scholar 

  • Neu R, Goffart S, Wolf K, Schäfer B (1998) Relocation of urf a from the mitochondrion to the nucleus cures the mitochondrial mutator phenotype in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 258:389–396

    Article  CAS  PubMed  Google Scholar 

  • Nunnari J, Walter P (1996) Regulation of organelle biogenesis. Cell 84:389–394

    CAS  PubMed  Google Scholar 

  • Nunnari J, Marshall WF, Straight A, Murray A, Sedat JW, Walter P (1997). Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA. Mol Biol Cell 8:1233–1242

    CAS  PubMed  Google Scholar 

  • Oakley BR, Rinehart JE (1985) Mitochondria and nuclei move by different mechanisms in Aspergillus nidulans. J Cell Biol 101:2392–2397

    CAS  PubMed  Google Scholar 

  • Oxelmark E, Marchini A, Malanchi I, Magherini F, Jaquet L, Hajibagheri MA, Blight KJ, Jauniaux JC, Tommasino M (2000) Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome. Mol Cell Biol 20:7784–7797

    Article  CAS  PubMed  Google Scholar 

  • Paluh JL, Clayton DA (1996) A functional dominant mutation in Schizosaccharomyces pombe RNase MRP RNA affects nuclear RNA processing and requires the mitochondrial-associated nuclear mutation ptp1-1 for viability. EMBO J 15:4723–4733

    CAS  PubMed  Google Scholar 

  • Paschke T, Meurer P, Schäfer B, Wolf K (1994) Homing of mitochondrial introns in the fission yeast Schizosaccharomyces pombe. Endocytobios Cell Res 10:205–213

    Google Scholar 

  • Pellenz S, Harrington A, Wolf K, Dujon B, Schäfer B (2002) Characterization of the I-SpomI endonuclease from fission yeast: insights into the evolution of a group I intron encoded homing endonuclease. J Mol Evol 55:302–313

    Article  CAS  PubMed  Google Scholar 

  • Pelloquin L, Belenguer P, Menon Y, Ducommun B (1998) Identification of a fission yeast dynamin-related protein involved in mitochondrial DNA maintenance. Biochem Biophys Res Commun 251:720–726

    Article  CAS  PubMed  Google Scholar 

  • Pelloquin L, Belenguer P, Menon Y, Gas N, Ducommun B (1999) Fission yeast Msp1 is a mitochondrial dynamin-related protein. J Cell Sci 112:4151–4161

    CAS  PubMed  Google Scholar 

  • Ropp PA, Copeland WC (1995) Characterization of a new DNA polymerase from Schizosaccharomyces pombe: a probable homologue of the Saccharomyces cerevisiae DNA polymerase gamma. Gene 165:103–107

    Article  CAS  PubMed  Google Scholar 

  • Sazer S, Sherwood SW (1990) Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast. J Cell Sci 97:509–516

    CAS  PubMed  Google Scholar 

  • Schäfer B, Wolf K (1998) The variable mitochondrial genome of the fission yeast Schizosaccharomyces pombe: novel group II introns. Endocytobios Cell Res 12:157–161

    Google Scholar 

  • Schäfer B, Wolf K (1999) A novel group II intron in the cox1 gene of the fission yeast Schizosaccharomyces pombe is inserted in the same codon as the mobile group II intron aI2 in the Saccharomyces cerevisiae cox1 homologue. Curr Genet 35:602–608

    Article  PubMed  Google Scholar 

  • Schäfer B, Merlos-Lange AM, Anderl C, Welser F, Zimmer M, Wolf K (1991) The mitochondrial genome of Schizosaccharomyces pombe: inability of all introns to splice autocatalytically, and construction of an intronless genome. Mol Gen Genet 225:158–167

    PubMed  Google Scholar 

  • Schäfer B, Wilde B, Massardo DR, Manna F, Del Giudice L, Wolf K (1994) A mitochondrial group-I intron in fission yeast encodes a maturase and is mobile in crosses. Curr Genet 25:336–341

    PubMed  Google Scholar 

  • Schäfer B, Kaulich K, Wolf K (1998) Mosaic structure of the cox2 gene in the petite negative yeast Schizosaccharomyces pombe: a group II intron is inserted at the same location as the otherwise unrelated group II introns in the mitochondria of higher plants. Gene 214:101–112

    Article  PubMed  Google Scholar 

  • Schäfer B, Gan L, Perlman PS (2003) Reverse transcriptase and reverse splicing activities encoded by the mobile group II Intron cobI1 of fission yeast mitochondrial DNA. J Mol Biol (in press)

  • Schmidt WM, Schweyen RJ, Wolf K, Mueller MW (1994) Transposable group II introns in fission and budding yeast. Site-specific genomic instabilities and formation of group II IVS plDNAs. J Mol Biol 243:157–166

    Article  CAS  PubMed  Google Scholar 

  • Seitz-Mayr G, Wolf K (1982) Extrachromosomal mutator inducing point mutations and deletions in mitochondrial genome of fission yeast. Proc Natl Acad Sci USA 79:2618–2622

    CAS  PubMed  Google Scholar 

  • Svoboda A, Slaninova I (1997) Colocalization of microtubules and mitochondria in the yeast Schizosaccharomyces japonicus var. versatilis. Can J Microbiol 43:945–953

    CAS  PubMed  Google Scholar 

  • Szczepanek T, Lazowska J (1996) Replacement of two non-adjacent amino acids in the S. cerevisiae bI2 intron-encoded RNA maturase is sufficient to gain a homing-endonuclease activity. EMBO J 15:3758–3767

    CAS  PubMed  Google Scholar 

  • Szczepanek T, Jamoussi K, Lazowska J (2000) Critical base substitutions that affect the splicing and/or homing activities of the group I intron bI2 of yeast mitochondria. Mol Gen Genet 264:137–144

    Article  CAS  PubMed  Google Scholar 

  • Trinkl H, Wolf K (1986) The mosaic cox1 gene in the mitochondrial genome of Schizosaccharomyces pombe: minimal structural requirements and evolution of group I introns. Gene 45:289–297

    Article  CAS  PubMed  Google Scholar 

  • Trinkl H, Wolf K (1989) Nucleotide sequence of the gene encoding subunit 3 of cytochrome c oxidase (cox3) in the mitochondrial genome of Schizosaccharomyces pombe strain EF1. Nucleic Acids Res 17:10104

    CAS  PubMed  Google Scholar 

  • Trinkl H, Lang BF, Wolf K (1985) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe 7.Continous gene for apocytochrome b in strain EF1 (CBS 356) and sequence variation in the region of intron insertion in strain ade7-50h −. Mol Gen Genet 198:360–363

    CAS  PubMed  Google Scholar 

  • Trinkl H, Lang BF, Wolf K (1989). Nucleotide sequence of the gene encoding the small ribosomal RNA in the mitochondrial genome of the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res 17:6730

    CAS  PubMed  Google Scholar 

  • White MF, Lilley DM (1997) Characterization of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe. Mol Cell Biol 17:6465–6471

    CAS  PubMed  Google Scholar 

  • White MF, Lilley DM (1998) Interaction of the resolving enzyme YDC2 with the four-way DNA junction. Nucleic Acids Res 26:5609–5616

    CAS  PubMed  Google Scholar 

  • Wolf K, Ahne A, Del Giudice L, Oraler G, Kanbay F, Merlos-Lange AM, Welser F, Zimmer M (1987) Introns as key elements in the evolution of mitochondrial genomes in lower eukaryotes. In: Papa S, Chance B, Ernster L (eds) Cytochrome systems. Plenum, New York, pp 119–127

  • Wong ED, Wagner JA, Scott SV, Okreglak V, Holewinske TJ, Cassidy-Stone A, Nunnari J (2003) The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion. J Cell Biol 160: 303–311

    Article  CAS  PubMed  Google Scholar 

  • Wool IG (1996) Extraribosomal functions of ribosomal proteins. Trends Biochem Sci 21:164–165

    Article  CAS  PubMed  Google Scholar 

  • Yaffe MP (1999) Dynamic mitochondria. Nat Cell Biol 1:E149–E150

    Google Scholar 

  • Yaffe MP, Harata D, Verde F, Eddison M, Toda T, Nurse P (1996) Microtubules mediate mitochondrial distribution in fission yeast. Proc Natl Acad Sci USA 93:11664–11668

    Article  CAS  PubMed  Google Scholar 

  • Yasuhira S, Yasui A (2000) Alternative excision repair pathway of UV-damaged DNA in Schizosaccharomyces pombe operates both in nucleus and in mitochondria. J Biol Chem 275:11824–11828

    CAS  PubMed  Google Scholar 

  • Zimmer M, Lückemann G, Lang BF, Wolf K (1984) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: 3. Gene mapping in strain EF1 (CBS 356) and analysis of hybrids between the strains EF1 and ade7-50h−. Mol Gen Genet 196:473–481

    CAS  PubMed  Google Scholar 

  • Zimmer M, Welser F, Oraler G, Wolf K (1987) Distribution of mitochondrial introns in the species Schizosaccharomyces pombe and the origin of the group II intron in the gene encoding apocytochrome b. Curr Genet 12:329–336

    CAS  PubMed  Google Scholar 

  • Zimmer M, Krabusch M, Wolf K (1991) Characterization of a novel reading frame urf a, in the mitochondrial genome of fission yeast: correlation of urf a mutations with a mitochondrial mutator phenotype and a possible role of frameshifting in urf a expression. Curr Genet 19:95–102

    CAS  PubMed  Google Scholar 

  • Zimmerly S, Hausner G, Wu X (2001) Phylogenetic relationships among group II intron ORFs. Nucleic Acids Res 29:1238–1250

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

Research in this laboratory was supported by the Deutsche Forschungsgemeinschaft.

Author information

Authors and Affiliations

  1. Department of Biology IV (Microbiology), Aachen Technical University, Worringer Weg, 52056, Aachen, Germany

    Bernd Schäfer

Authors
  1. Bernd Schäfer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bernd Schäfer.

Additional information

Communicated by M. Brunner

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schäfer, B. Genetic conservation versus variability in mitochondria: the architecture of the mitochondrial genome in the petite-negative yeast Schizosaccharomyces pombe . Curr Genet 43, 311–326 (2003). https://doi.org/10.1007/s00294-003-0404-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00294-003-0404-5

Keywords

Search

Navigation