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Immunhistochemische Darstellung von Lymphgefäßen mit D2–40 in der diagnostischen Pathologie

Immunohistochemical identification of lymph vessels with D2–40 in diagnostic pathology

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Zusammenfassung

Mit D2–40 ist ein Antikörper verfügbar, der Lymphgefäßendothelien des Menschen verlässlich markiert. Wir haben eine orientierende Studie an verschiedenen pathologischen und nichtpathologischen Geweben durchgeführt, um herauszufinden, ob und welche anderen Zellen D2–40 exprimieren.

Durchgängig werden normale und reaktive Lymphgefäße sowie Lymphgefäßtumoren markiert. Auch ein Teil der Angiosarkome und das Kaposi-Sarkom sind D2–40-positiv.

Positiv reagierende nichtendothelialen Zellen: Mesothelien, follikuläre dendritische Retikulumzellen, interstitielle Zellen von Cajal, einzelne Epithelien der Epidermis und einzelner Hautanhangsgebilde sowie Myoepithelien (z. B. Mamma), basale Epithelien (z. B. Prostata) und verschiedene mesenchymale Zellen.

Positiv reagierende Tumoren: myofibroblastische Tumoren, der gastrointestinale Stromatumor, Mesotheliome, der Adenomatoidtumor, traumatische Neurome, Seminome, das Seminoma in situ und andere Hodentumoren, zweimal ein Karzinom, ein pleomorphes Adenom und das Sarkom der follikulären dendritischen Retikulumzellen.

Trotz des relativ breiten Reaktionsspektrums ist D2–40 der nach unserer Einsicht derzeit am besten geeignete und zudem kommerziell erhältliche Antikörper zur Darstellung normaler, reaktiver und neoplastischer Lymphgefäßendothelien.

Abstract

D2–40 is an antibody that reliably detects human lymphatic endothelial cells. Because little is known about the extent to which other cells are stained, we have investigated this question in various tissues.

Normal, reactive and neoplastic lymphatics (including lymphangiomas and Stewart-Treves syndrome) were stained by D2–40. Published findings indicate that some angiosarcomas and Kaposi are positive. Staining was also found in the following non-endothelial cells: mesothelial cells, follicular dendritic reticulum cells, Cajal cells, a few epithelial cells in the dermis, myoepithelial cells (e. g. breast), and basal cells (e. g. prostata), various mesenchymal cells. The following tumours were reactive: myofibroblastic tumours, gastrointestinal stromal tumour, mesothelioma, adenomatoid tumour, traumatic neuroma, seminoma, seminoma in situ and other testicular tumours, pleomorphic adenoma (one of two tumours positive), and follicular dendritic reticulum cell sarcoma.

Despite this relatively broad spectrum of reactivity, we consider D2–40 to be the best commercially available antibody for the delineation of normal, reactive and neoplastic lymphatic endothelial cells.

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Danksagung

Ich danke bei dieser Gelegenheit in besonderem Maße meiner MTA Frau Anne Adam für ihr langjähriges und das übliche Maß weit überschreitende Engagement. In diesen Dank eingebunden sind zahlreiche weitere Personen, meine Sekretärin Frau Dorothea Biesinger, Frau Adelheid Mall, Frau Friederike Müller, Frau Carola Weihing und Frau Brigitte Bachmann mit ihren zahlreichen Mitarbeitern/innen.

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  1. Institut für Pathologie, Universitätsklinikum, Eberhard-Karls-Universität Tübingen

    E. Kaiserling

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Kaiserling, E. Immunhistochemische Darstellung von Lymphgefäßen mit D2–40 in der diagnostischen Pathologie. Pathologe 25, 362–374 (2004). https://doi.org/10.1007/s00292-004-0693-6

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