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Natural gum as bio-reductant to green synthesize silver nanoparticles: assessing the apoptotic efficacy on MCF-7 and SH-SY5Y cell lines and their antimicrobial potential

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Abstract

In the present study, silver nanoparticles (AgNPs) were synthesized using Prunus avium gum extract and their physicochemical properties together with antibacterial and cytotoxic effects were evaluated. The characterization of these AgNPs was performed by UV–vis spectroscopy, transmission electron microscopy, scanning electron microscopy, and Fourier transforms infrared spectroscopy. The bio-performance of the nanoparticles was also evaluated. Our results exhibit the prominent antibacterial activity of AgNPs against both Gram-positive and Gram-negative bacteria. The MTT results indicate cytotoxicity of AgNPs on MCF-7 and SH-SY5Y cell lines at 50 and 10 µg mL−1 concentrations, respectively. Moreover, gene expression analysis by real-time PCR represents the upregulation of Bax mRNA and downregulation of Bcl-2 mRNA, demonstrates the induction of apoptosis after AgNPs treatment. These findings imply that green-synthesized AgNPs could be a promising candidate in biomedical applications such as battling cancer and infectious diseases.

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The scheme of preparation of AgNPs and its effect on cell and bacteria.

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Acknowledgements

The authors acknowledge the financial support for this investigation by the Research Council of Baqiyatallah University of Medical Sciences (Tehran, Iran) and Shahid Bahonar University (Kerman, Iran).

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Authors and Affiliations

  1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mohammad Hashemabadi & Ramezan Ali Taheri

  2. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

    Hosseinali Sasan

  3. Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran

    Mojdeh Amandadi

  4. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

    Reza Mohammadinejad

  5. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Gholamreza Farnoosh

  6. Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, PO Box 89195-999, Yazd, Iran

    Mostafa Azimzadeh

  7. Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, PO Box 89195-999, Yazd, Iran

    Mostafa Azimzadeh

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  1. Mohammad Hashemabadi
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Hashemabadi, M., Sasan, H., Amandadi, M. et al. Natural gum as bio-reductant to green synthesize silver nanoparticles: assessing the apoptotic efficacy on MCF-7 and SH-SY5Y cell lines and their antimicrobial potential. Polym. Bull. 78, 2867–2886 (2021). https://doi.org/10.1007/s00289-020-03238-9

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  • DOI: https://doi.org/10.1007/s00289-020-03238-9

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