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Characterization of thermo-sensitive hydrogels based on poly(N-isopropylacrylamide)/hyaluronic acid

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Abstract

In this study, an hydrogel was synthesized from the monomer N-isopropylacrylamide (NIPA), generating the poly(N-isopropylacrylamide) (PNIPA) and other formulations were synthesized in the presence of 1, 2, and 3% hyaluronic acid (HA) for obtain an interpenetrating polymer network. For all the obtained hydrogels, the thermo-sensitive response was studied since the lower critical solution temperature (LCST) and was analyzed by differential scanning calorimetry (DSC), nuclear magnetic resonance (NMR), and oscillatory rheology at constant frequency as a function of temperature. The LCST transition temperature (Tt onset) was found between 34.4 and 35.5 °C. By scanning electron microscopy (SEM) PNIPA-HA formulations showed a porous morphology. The applicability of the hydrogels as injectable and non-toxic materials was verified, respectively, by rheology results and by cytotoxicity studies through an in vitro test of cell hemolysis on blood agar.

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Acknowledgments

The authors thank Professor Alejandro J. Müller (Head of USB Polymer Group GPUSB-1, Department of Materials Science, Universidad Simón Bolívar) for providing fructiferous discussions on the results here presented and for to use some equipments to characterize these samples. Also, thanks to the Decanato de Investigación y Desarrollo (DID) for their financial support through the Fund-PPI. Also thank for support from thematic IberoAmerican network BIOFAB (Biofabricação: Materiais, Processes e Simulação) funded by CYTED.

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Authors and Affiliations

  1. B5IDA Group, Department of Chemistry, Universidad Simón Bolívar, Apartado 89000, Caracas, 1080, Venezuela

    Ramón Coronado & Marcos A. Sabino

  2. IVIC, Venezuelan Institute of Scientific Research, Chemistry Center, Altos de Pipe, Estado Miranda, Venezuela

    Sara Pekerar

  3. USB Polymer Group, Department of Materials Science, Universidad Simón Bolívar, Apdo. 89000, Caracas, 1080, Venezuela

    Arnaldo T. Lorenzo

Authors
  1. Ramón Coronado
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  2. Sara Pekerar
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  3. Arnaldo T. Lorenzo
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  4. Marcos A. Sabino
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Correspondence to Marcos A. Sabino.

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Coronado, R., Pekerar, S., Lorenzo, A.T. et al. Characterization of thermo-sensitive hydrogels based on poly(N-isopropylacrylamide)/hyaluronic acid. Polym. Bull. 67, 101–124 (2011). https://doi.org/10.1007/s00289-010-0407-6

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  • DOI: https://doi.org/10.1007/s00289-010-0407-6

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