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Cloning of Two New cry Genes from Bacillus thuringiensis subsp. wuhanensis Strain

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Abstract.

With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8–78.7% identical to that of Cry1B group. The nomenclature assignment of these two clones is, therefore, named Cry1Gb1 and Cry1Bd1, respectively. The Cry1Bd1 is toxic to Plutella xylostella larvae, and the Cry1Gb1 is toxic to Pieris rapae larvae.

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Authors and Affiliations

  1. Institute of Biochemistry, National Yang Ming University, Shih-Pai, Taipei, Taiwan 11221, Republic of China , , , , , , TW

    White-Shang Kuo, Jong-Huon Lin & Kin-Fu Chak

  2. Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Taichung Hsien 41301, Republic of China , , , , , , TW

    Ching-Chou Tzeng & Shui-Shang Kao

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  1. White-Shang Kuo
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  2. Jong-Huon Lin
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  3. Ching-Chou Tzeng
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  4. Shui-Shang Kao
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  5. Kin-Fu Chak
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Received: 2 August 1999 / Accepted: 18 October 1999

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Kuo, WS., Lin, JH., Tzeng, CC. et al. Cloning of Two New cry Genes from Bacillus thuringiensis subsp. wuhanensis Strain. Curr Microbiol 40, 227–232 (2000). https://doi.org/10.1007/s002849910046

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  • DOI: https://doi.org/10.1007/s002849910046

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