Abstract.
We analyzed the pathogenicity of chitin synthetase (chs) disruptants of Ustilago maydis obtained with the carboxin-resistant or the hygromycin-resistant cassettes. We found that only chitin synthetase (chs) mutants obtained by gene disruption with the carboxin resistance cassette lost their virulence to maize (Zea mays) seedlings. Carboxin is a systemic fungicide that inhibits respiration by preventing the oxidation of succinate. We demonstrated that carboxin-resistant transformants were affected in the levels of succinate dehydrogenase and respiratory activities when compared with hygromycin-resistant disruptants. We propose that loss of virulence in the carboxin-resistant transformants is owing to loss of respiratory fitness, which probably represents an important component of virulence in this fungus.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 13 May 1999 / Accepted: 26 June 1999
Rights and permissions
About this article
Cite this article
Ruiz-Herrera, J., Martínez-Espinoza, A., Alvarez, P. et al. Carboxin-Resistant Mutant of Ustilago maydis Is Impaired in Its Pathogenicity for Zea mays . Curr Microbiol 39, 291–294 (1999). https://doi.org/10.1007/s002849900461
Issue Date:
DOI: https://doi.org/10.1007/s002849900461